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Abstract

Wood cellulose into fuel ethanol is the research focus in recent years. This paper studies the wood cellulose enzyme production of fuel ethanol process optimization, the focus is to improve efficiency and reduce enzyme cellulase costs, specifically including the following:
Cellulase Characterization Study: through a variety of cellulose and hemicellulose enzyme activity of filter paper, fiber and protein activity of sugar content, the comparison of the performance.
Fu optimize the allocation of more of the enzyme research: In view of the complexity of wood cellulose, examining the adjustment of the type of enzyme in the enzyme effect change. Corn cob and straw in the system, compared the effects of two cellulose enzyme, the research in the cellulose fibers and the sugar-added pectinase or xylanase on the hydrolysis of cellulose and hemicellulose efficiency Implications, as well as different xylanase enzyme hydrolysis of the effect of the changes. The results showed that: multi-fu match the effects of the enzyme substrate types, the enzyme used by the species and quantity of the impact. Pectinase than xylanase better, less pectinase to a large extent, improve the efficiency of semi-cellulose hydrolysis.
Of the recovery: In order to improve efficiency in the use of enzymes, the use of lower-cost, the paper for the recycling and reuse of a study. Discussed membrane separation, fixed and re-absorption of three methods of recycling and re-absorption to try to do the test, the pilot of the recovery has been relatively good results.
Key words: wood cellulose, the enzyme efficiency, activity, with multi-fu, of recovery
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