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[求助]
NADH还原型辅酶I在什么Buffer中保存稳定？
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我是学化学的，对生物不是很了解，最近在查阅NADH还原型辅酶I的保存Buffer的资料，看到以前的帖子有说用tris buffer PH~8的，帖子里也贴有文献， 但是只是没有文献出处，想请教各位生物高手，能否告知这篇文献的出处，或者发给我这篇文献？ 另外只说了可用tris buffer PH~8，那么buffer浓度是多少呢？配制酶的浓度又是多少呢，还是酶的浓度是根据要测定的东西来定，比如我们要测定ALT，AST的， 那么NADH的浓度应该是多少呢？希望各位多多指点，万分感谢！ 文献内容如下： Colowick, Kaplan, and Ciotti (4) observed that the rate of hydrolysis of the nicotinamide-ribose linkage of NAD at 100” increases with increasing pH above pH 6.0. The rates of hy- drolysis obtained in various buffers at pH 7.5 were found to de- pend on the type of buffer used. Tris and acetate buffers did not increase the rate of hydrolysis above that observed with un- buffered NAD at this pH, whereas phosphate and citrate buffers enhanced it 3- to 4-fold. It was noted (1) that Tris could almost completely abolish this stimulatory effect of phosphate and citrate buffers |
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