24小时热门版块排行榜

返回列表

孔孔

金虫 (小有名气)

应助: 0 (幼儿园)
金币: 346.8
红花: 1
帖子: 211
在线: 72.1小时
虫号: 236087
注册: 2006-04-02
性别: MM
专业: 有机合成

[求助] NADH还原型辅酶I在什么Buffer中保存稳定？

我是学化学的，对生物不是很了解，最近在查阅NADH还原型辅酶I的保存Buffer的资料，看到以前的帖子有说用tris buffer PH~8的，帖子里也贴有文献，
但是只是没有文献出处，想请教各位生物高手，能否告知这篇文献的出处，或者发给我这篇文献？
另外只说了可用tris buffer PH~8，那么buffer浓度是多少呢？配制酶的浓度又是多少呢，还是酶的浓度是根据要测定的东西来定，比如我们要测定ALT，AST的，
那么NADH的浓度应该是多少呢？希望各位多多指点，万分感谢！
文献内容如下：
Colowick,  Kaplan,  and  Ciotti  (4)  observed  that  the  rate  of
hydrolysis  of  the  nicotinamide-ribose  linkage  of  NAD  at  100”
increases  with  increasing  pH  above  pH  6.0.  The  rates  of  hy-
drolysis  obtained  in  various  buffers  at  pH  7.5  were  found  to  de-
pend  on  the  type  of  buffer  used.  Tris  and  acetate  buffers  did
not  increase  the  rate  of  hydrolysis  above  that  observed  with  un-
buffered  NAD  at  this  pH,  whereas  phosphate  and  citrate  buffers
enhanced  it  3-  to  4-fold.  It  was  noted  (1)  that  Tris  could  almost
completely  abolish  this  stimulatory  effect  of  phosphate  and
citrate  buffers

回复此楼