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小木虫论坛-学术科研互动平台 » 生物医药区 » 微生物 » 实验/技术 » How to analyse the non-reducing products after acid hydrolysis of corn stover?
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c1loh1la

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[求助] How to analyse the non-reducing products after acid hydrolysis of corn stover? 已有1人参与

After using acid to hydrolysis the corn stover, I have got a variety of carbohydrates, including a lot of polysaccharide which can not be analyse by DNS method, which process should I use to make these non-reducing products measurable by DNS? would Adding 4% H2SO4 and heated in 121℃ for 1 hour do the trick? or is there a better plan?

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1楼 2015-03-05 16:04:09
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冼亮淀粉酶

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【答案】应助回帖

感谢参与，应助指数 +1
why do not you use the HPLC method with those sugars likely present as standards? and you can chose the eluent according to those references. i strongly recommend you the NH2 or sugar column.
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2楼2015-03-07 00:25:09
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冼亮淀粉酶

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A wealth of literature already exist on analysis of sugars. please use HPLC 一般人我都会告诉TA
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3楼2015-03-07 00:29:50
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