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c1loh1la

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[求助] How to analyse the non-reducing products after acid hydrolysis of corn stover? 已有1人参与

After using acid to hydrolysis the corn stover, I have got a variety of carbohydrates, including a lot of polysaccharide which can not be analyse by DNS method, which process should I use to make these non-reducing products measurable by DNS? would Adding 4% H2SO4 and heated in 121℃ for 1 hour do the trick? or is there a better plan?

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冼亮淀粉酶

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生物大分子降解酶

【答案】应助回帖

感谢参与,应助指数 +1
why do not you use the HPLC method with those sugars likely present as standards? and you can chose the eluent according to those references. i strongly recommend you the NH2 or sugar column.
流星来时我许了个愿,愿我有吃有喝还有舒服的猪圈。讨厌发帖前不搜索论坛重复发帖和仅把论坛当解决问题工具,久不看论坛一来就提问者,宁弃EPI和VIP也不回帖。
2楼2015-03-07 00:25:09
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冼亮淀粉酶

专家顾问 (知名作家)

生物大分子降解酶

A wealth of literature already exist on analysis of sugars. please use HPLC 一般人我都会告诉TA
流星来时我许了个愿,愿我有吃有喝还有舒服的猪圈。讨厌发帖前不搜索论坛重复发帖和仅把论坛当解决问题工具,久不看论坛一来就提问者,宁弃EPI和VIP也不回帖。
3楼2015-03-07 00:29:50
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