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HARRY231木虫 (小有名气)
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应用样品的前处理新技术SPE结合GC/MS的系统分析方法，建立了农药的多残留分析方法。利用不同极性的固相萃取小柱，同时提取净化生物样品血液中10种农药和鼠药，并利用GC/MS监测分析将保留时间、特征离子和谱库检索相结合进行多指标检测。提取过程中充分考虑了固相萃取的可能影响因素，对SPE萃取条件进行优化，选择出了样品提取的最优方案。利用三种不同极性的SPE小柱提取净化效果比较得知，HLB柱的萃取效果最佳；从氯仿、苯、乙酸乙酯及氯仿：异丙醇（9：1）不同的洗脱剂种类比选后得知，洗脱剂为氯仿，洗脱剂的用量定位3mL。加入2mL磷酸盐缓冲液时效果最佳。结果表明该方法系统性好，操作简便，具有较高的灵敏度、准确度和精密度。添加回收率在72.6%~112%之间，变异系数均小于 ，完全符合农药残留提取方法要求。 谢谢！ [ Last edited by HARRY231 on 2008-6-11 at 12:09 ] |
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HARRY231(金币+1,VIP+0):谢谢您的回复，但我需要的不是直接google的结果。
HARRY231(金币+1,VIP+0):谢谢您的回复，但我需要的不是直接google的结果。
| Application of the sample pre-treatment with the new technology SPE GC / MS analysis of the way, the establishment of a multi-pesticide residue analysis. The use of different polarity by SPE column, and purification of biological samples from the blood of 10 pesticides and rat poison, and using GC / MS monitoring and analysis will be retained, the spectral characteristics of ions and retrieval of combining multi-target detection. Give full consideration to the process of extracting the solid phase extraction and the possible effects of factors, the SPE extraction conditions to optimize and select a sample from the optimal programme. Use of three different polarity of the SPE column purifying effect from that comparison, HLB, in the extraction of the best results, from chloroform, benzene, chloroform and ethyl acetate: isopropyl alcohol (9:1) of the wash Delinking of species than that after the election, to get rid of chloroform, get rid of the targeted amount of 3 mL. Adding 2 mL phosphate buffer at the best results. The results showed that the systemic approach, and simple, has a high sensitivity, accuracy and precision. Add in the recovery of 72.6 percent to 112 percent, and the coefficient of variation was less than full compliance with the requirements of pesticide residue extraction methods. |
2楼2008-06-11 14:19:16
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