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zhouzhuxin木虫 (著名写手)
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[交流]
求MOPs 缓冲液的全面介绍
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| MOPs缓冲液能否在细胞中用？ |
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zhouzhuxin(金币+5,VIP+0): 4-27 23:37
zhouzhuxin(金币+5,VIP+0): 4-27 23:37
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以下是AMRESCO对MOPS的说明，请参考 Biological buffers such as MOPS allow the pH of an aqueous solution to remain constant while the concentration of hydrogen ions present changes. These buffers have pKa values at or near physiological pH. Biological buffers are non-toxic to cells, and are not absorbed through cell membranes. The concentration, temperature, and ionic composition of the medium has minimal affect on the buffering capacity. These buffers are resistant to enzymatic and nonenzymatic degradation furthermore, they are essentially transparent to visible and ultraviolet light. MOPS is used for RNA electrophoresis in agarose. It is available in a free acid and sodium salt form, and works exceptionally well for formaldehyde gels at a 20mM concentration. The free acid form must be adjusted to the working pH with an appropriate base such as sodium hydroxide, potassium hydroxide, or tetramethylammonium hydroxide. MOPS 10X liquid concentrate is prepared with diethylpyrocarbonate (DEPC) treated water, leaving the solution RNase free. References: Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Strohl, eds. Current Protocols in Molecular Biology. John Wiley and Sons, New York. 1994. p.p. 4.9.1, 9.1.1. Good, N.E., G.D. Winget, W. Winter, T.N. Connolly, K. Izana, and R.M.M. Singh. 1966. Biochemistry 5:467. Shipman, C. In Tissue Culture Methods and Applications. P.F. Kruse and M.K. Patterson, Eds. Academic Press, New York. 1973. [ Last edited by xipha on 2008-9-3 at 22:38 ] |
2楼2008-09-03 22:35:22
zhouzhuxin
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3楼2008-12-03 14:49:48