| 查看: 494 | 回复: 2 | |||
| 当前主题已经存档。 | |||
zhouzhuxin木虫 (著名写手)
|
[交流]
求MOPs 缓冲液的全面介绍
|
||
| MOPs缓冲液能否在细胞中用？ |
回复此楼» 猜你喜欢
售SCI一区T0P文章，我:8O.55.1.O.5.4,科目齐全,可+急
已经有5人回复
-
售SCI-T0P文章，我:8O.5.5.1.O.54,科目齐全,可+急
已经有7人回复
-
上海工程技术大学 激光智能制造课题组 2027级博士研究生招生
已经有6人回复
-
课题组招2027级博士 上海工程技术大学 激光智能制造方向
已经有6人回复
-
现代”学阀”该如何界定
已经有14人回复
-
师弟论文见刊大半年才想起来申请专利，还能抢救一下吗？
已经有4人回复
-
有机合成以后会不会被AI改变？做科研的虫友怎么看
已经有8人回复
-
我的奶奶
已经有3人回复
★ ★ ★ ★ ★
zhouzhuxin(金币+5,VIP+0): 4-27 23:37
zhouzhuxin(金币+5,VIP+0): 4-27 23:37
|
以下是AMRESCO对MOPS的说明，请参考 Biological buffers such as MOPS allow the pH of an aqueous solution to remain constant while the concentration of hydrogen ions present changes. These buffers have pKa values at or near physiological pH. Biological buffers are non-toxic to cells, and are not absorbed through cell membranes. The concentration, temperature, and ionic composition of the medium has minimal affect on the buffering capacity. These buffers are resistant to enzymatic and nonenzymatic degradation furthermore, they are essentially transparent to visible and ultraviolet light. MOPS is used for RNA electrophoresis in agarose. It is available in a free acid and sodium salt form, and works exceptionally well for formaldehyde gels at a 20mM concentration. The free acid form must be adjusted to the working pH with an appropriate base such as sodium hydroxide, potassium hydroxide, or tetramethylammonium hydroxide. MOPS 10X liquid concentrate is prepared with diethylpyrocarbonate (DEPC) treated water, leaving the solution RNase free. References: Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Strohl, eds. Current Protocols in Molecular Biology. John Wiley and Sons, New York. 1994. p.p. 4.9.1, 9.1.1. Good, N.E., G.D. Winget, W. Winter, T.N. Connolly, K. Izana, and R.M.M. Singh. 1966. Biochemistry 5:467. Shipman, C. In Tissue Culture Methods and Applications. P.F. Kruse and M.K. Patterson, Eds. Academic Press, New York. 1973. [ Last edited by xipha on 2008-9-3 at 22:38 ] |
2楼2008-09-03 22:35:22
zhouzhuxin
木虫 (著名写手)
- 应助: 17 (小学生)
- 金币: 3663.9
- 散金: 416
- 红花: 3
- 帖子: 1110
- 在线: 216.8小时
- 虫号: 475733
- 注册: 2007-12-07
- 性别: MM
- 专业: 化学环境污染与健康
3楼2008-12-03 14:49:48