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Nice melting curve. For no stationary phase: 1. Increase PCR cycle 2. Decrease sample dilution. Are you working with cDNA/gDNA/plasmid/RNA/PCR fragment as template? 3. You said if you increase you PCR cycle, there will be some nonspecific amplification. If this is true, stop trying anything, redesign your primers and be sure there is no non-specific amplification! But your melting curve didn't show any signs of non-specificity! Any explanations? |

10Â¥2015-01-16 00:47:46
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2Â¥2015-01-15 13:02:54
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3Â¥2015-01-15 13:29:50
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4Â¥2015-01-15 13:30:31














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