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yinxiaoming817金虫 (著名写手)
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10金翻译下面摘要啊!(汉译英)
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重金翻译下面摘要啊! 本文设计的是一种新型微井阵列式PCR芯片，此芯片可完成DNA样品的扩增过程和扩增产物的电化学检测过程。芯片整体尺寸为1cm×1cm×1mm，其上面有5×5阵列式微井。微井以多晶硅为衬底由SU8胶制作，每个微井的尺寸为1mm×1mm×200um。 微加热器和温度微传感器嵌在多晶硅衬底上。每一排的微加热器形貌相同，但不同排之间保持不同的形貌，以此研究不同形貌的微加热器在PCR扩增温度循环过程中加热性能的差异。用于电化学检测的微电极做在上方的硅盖上，除第一列保持相同形貌以研究微加热器的性能外，其它每一排的微电极都有所变化，如将工作电极做成“竖琴”状、 “梳子”状、变化工作电极和辅助电极之间的距离、变化工作电极的面积，以此来研究电极的某些变化因素对目标DNA电化学检测的影响。 谢谢.......... |
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4楼2008-05-29 19:38:49
yinxiaoming817
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2楼2008-05-29 16:35:52
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yinxiaoming817(金币+3,VIP+0):辛苦了,谢谢!
yinxiaoming817(金币+3,VIP+0):辛苦了,谢谢!
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本文设计的是一种新型微井阵列式PCR芯片，此芯片可完成DNA样品的扩增过程和扩增产物的电化学检测过程。芯片整体尺寸为1cm×1cm×1mm，其上面有5×5阵列式微井。微井以多晶硅为衬底由SU8胶制作，每个微井的尺寸为1mm×1mm×200um。 微加热器和温度微传感器嵌在多晶硅衬底上。每一排的微加热器形貌相同，但不同排之间保持不同的形貌，以此研究不同形貌的微加热器在PCR扩增温度循环过程中加热性能的差异。 用于电化学检测的微电极做在上方的硅盖上，除第一列保持相同形貌以研究微加热器的性能外，其它每一排的微电极都有所变化，如将工作电极做成“竖琴”状、 “梳子”状、变化工作电极和辅助电极之间的距离、变化工作电极的面积，以此来研究电极的某些变化因素对目标DNA电化学检测的影响。 In this paper, the design is a new well array PCR chip, this chip can complete the expansion process of DNA samples and the electrochemical detection process. of amplified product .The overall chip size is 1 cm × 1cm × 1mm, its surface has a 5 × 5 array micro wells. Micro-wells are produced by the SU8 adhesive with polysilicon substrate ,the size of each micro-wells is 1 mm × 1mm × 200um.. Micro-heater and temperature sensors embedded in the polysilicon substrate. Each row of the morphology of micro-heateare are the same ,but different row keeps different topography, the difference of heating performance in micro-heater of different morphology in the process of PCR amplification temperature cycle was studied. The microelectrode used for electro chemical detection was made at the top of polysilicon substrate ,with the exception of the first row maintained the same shape to study the performance of micro-heater, every other row microelectrodes all have changes, such as making working electrodes "Harp"-like, "comb"-like, changing the distance between the working electrode and the auxiliary electrodes,and changing the area of working electrode .so as to study the effects of the electrodes of some changes on the detection electrochemical of target DNA. |
3楼2008-05-29 19:36:54
qqcor
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★ ★ ★ ★ ★
yinxiaoming817(金币+5,VIP+0):不错啊!辛苦了!谢谢!
yinxiaoming817(金币+5,VIP+0):不错啊!辛苦了!谢谢!
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What this article designs is one kind of new micro well array type PCR chip, this chip may complete the DNA sample to increase the process and to increase the product the electrochemistry examination process. The chip overall size for 1cm×1cm×1mm, its above has 5×5 the array to decline the well. The micro well manufactures take the polycrystalline silicon as the substrate by the SU8 rubber, each micro well size for 1mm×1mm×200um. The micro heater and the temperature micro sensor inlay on the polycrystalline silicon substrate. Each row of micro heater appearance is the same, but between the different platoon maintains the different appearance, by this research different appearance micro heater, in PCR increases in the temperature cycle process to heat up the performance the difference. Uses in the electrochemistry examination the micro electrode doing in the place above silicon covers, maintains besides the first row the same appearance studies the micro heater's performance, other each row of micro electrode has the change, if the working electrode will make “the harp” the shape, “the comb” the shape, between the change working electrode and auxiliary electrode's distance, the change working electrode's area, studies electrode's certain change factor by this to the goal DNA electrochemistry examination influence. |
5楼2008-05-29 19:55:30
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