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作者：Cai X D, Wang G Y, Cao W J. 题目：In Vitro Induction and Proliferation of Callus from Immature Cotyledons and Embryos of Juglans Regia cv. ‘Xiangling’ 期刊：Notulae Botanicae Horti Agrobotanici Cluj-Napoca 时间及页码： 2013, 41(2):378-384 [ Last edited by citrus276 on 2014-12-15 at 10:20 ] |
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In vitro Induction and Proliferation of Callus from Immature Cotyledons and Embryos of Juglans regia cv. 'Xiangling' 作者:Cai, XD (Cai, Xiao-Dong)[ 1 ] ; Wang, GY (Wang, Gui-Yuan)[ 1 ] ; Cao, WJ (Cao, Wen-Juan)[ 1 ] NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA 卷: 41 期: 2 页: 378-384 出版年: JUL-DEC 2013 查看期刊信息 摘要 Callus holds great potential for biotechnology applications in plants. In this study, several experiments were performed to establish a protocol for in vitro induction of good quality callus by using immature cotyledons and embryos of Asians regia cv. 'Xiangling' as the explants. DKW basal media supplemented with different concentrations of plant growth regulators were applied to determine the induction rate, texture and color of the recovered callus. Results showed that the optimum culture medium for the callus induction from these explants was a DKW basal medium consisting of I mg/L BA, 2 mg/L KT 250 mg/L glutamine, 500 mg/L casein hydrolysate, 200 mg/L Vc, 50 g/L sucrose and 2.8 g/L Gelrite (i.e., the T8 medium). The average rate of callus induction on the medium reached 80.7% for the immature cotyledons and 66.7% for the immature embryos, and all the callus was characterized by a friable, creamy or yellow appearance. In vitro proliferation of callus was not very successful as a result of the fact that the friable callus with creamy or yellow color grew very slowly, whereas the fast-growing callus was easy to turn brown and died eventually. Even though further investigation will be required to overcome the problem of browning and assess the regeneration ability of the callus, this approach for the production of friable callus may have valuable applications for walnut and other woody trees. 关键词 作者关键词:browning; callus induction rate; somatic embryogenesis; tissue; culture; walnut KeyWords Plus:EASTERN BLACK-WALNUT; SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; PROPAGATION; GERMINATION; OVARIES; L. 作者信息 通讯作者地址: Cai, XD (通讯作者) Yangtze Univ, Coll Hort & Gardening, Jinzhou, Hubei, Peoples R China. 地址: [ 1 ] Yangtze Univ, Coll Hort & Gardening, Jinzhou, Hubei, Peoples R China 电子邮件地址:caixiao.dong@163.com 基金资助致谢 基金资助机构 授权号 Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization 20011BLKF239 National Natural Science Foundation of China 30900979 查看基金资助信息 出版商 UNIV AGR SCI & VETERINARY MED CLUJ-NAPOCA, 3-5 MANASTUR ST, CLUJ-NAPOCA, 400372, ROMANIA 类别 / 分类 研究方向  lant SciencesWeb of Science 类别 lant Sciences文献信息 文献类型:Article 语种:English 入藏号: WOS:000328438300007 ISSN: 0255-965X 期刊信息 Impact Factor (影响因子): Journal Citation Reports® 其他信息 IDS 号: 272CQ Web of Science 核心合集中的 "引用的参考文献": 31 Web of Science 核心合集中的 "被引频次": 0 |
2楼2014-12-15 10:31:00
