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×÷ÕߣºCai X D, Wang G Y, Cao W J. ÌâÄ¿£ºIn Vitro Induction and Proliferation of Callus from Immature Cotyledons and Embryos of Juglans Regia cv. ¡®Xiangling¡¯ ÆÚ¿¯£ºNotulae Botanicae Horti Agrobotanici Cluj-Napoca ʱ¼ä¼°Ò³Â룺 2013, 41(2):378-384 [ Last edited by citrus276 on 2014-12-15 at 10:20 ] |
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citrus276: ½ð±Ò+40, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, thanks 2014-12-15 10:35:27
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oven1986: LS-EPI+1, ¸ÐлӦÖú¡£ 2014-12-15 21:13:35
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In vitro Induction and Proliferation of Callus from Immature Cotyledons and Embryos of Juglans regia cv. 'Xiangling' ×÷Õß:Cai, XD (Cai, Xiao-Dong)[ 1 ] ; Wang, GY (Wang, Gui-Yuan)[ 1 ] ; Cao, WJ (Cao, Wen-Juan)[ 1 ] NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA ¾í: 41 ÆÚ: 2 Ò³: 378-384 ³ö°æÄê: JUL-DEC 2013 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª Callus holds great potential for biotechnology applications in plants. In this study, several experiments were performed to establish a protocol for in vitro induction of good quality callus by using immature cotyledons and embryos of Asians regia cv. 'Xiangling' as the explants. DKW basal media supplemented with different concentrations of plant growth regulators were applied to determine the induction rate, texture and color of the recovered callus. Results showed that the optimum culture medium for the callus induction from these explants was a DKW basal medium consisting of I mg/L BA, 2 mg/L KT 250 mg/L glutamine, 500 mg/L casein hydrolysate, 200 mg/L Vc, 50 g/L sucrose and 2.8 g/L Gelrite (i.e., the T8 medium). The average rate of callus induction on the medium reached 80.7% for the immature cotyledons and 66.7% for the immature embryos, and all the callus was characterized by a friable, creamy or yellow appearance. In vitro proliferation of callus was not very successful as a result of the fact that the friable callus with creamy or yellow color grew very slowly, whereas the fast-growing callus was easy to turn brown and died eventually. Even though further investigation will be required to overcome the problem of browning and assess the regeneration ability of the callus, this approach for the production of friable callus may have valuable applications for walnut and other woody trees. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:browning; callus induction rate; somatic embryogenesis; tissue; culture; walnut KeyWords Plus:EASTERN BLACK-WALNUT; SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; PROPAGATION; GERMINATION; OVARIES; L. ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Cai, XD (ͨѶ×÷Õß) Yangtze Univ, Coll Hort & Gardening, Jinzhou, Hubei, Peoples R China. µØÖ·: [ 1 ] Yangtze Univ, Coll Hort & Gardening, Jinzhou, Hubei, Peoples R China µç×ÓÓʼþµØÖ·:caixiao.dong@163.com »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization 20011BLKF239 National Natural Science Foundation of China 30900979 ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ UNIV AGR SCI & VETERINARY MED CLUJ-NAPOCA, 3-5 MANASTUR ST, CLUJ-NAPOCA, 400372, ROMANIA Àà±ð / ·ÖÀà Ñо¿·½Ïò lant SciencesWeb of Science Àà±ð lant SciencesÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000328438300007 ISSN: 0255-965X ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: 272CQ Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 31 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 |

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