| ²é¿´: 1737 | »Ø¸´: 3 | ||||||
lxg133298ÈÙÓþ°æÖ÷ (ÎÄѧ̩¶·)
Stay Hungry. Stay Foolish.
|
[½»Á÷]
[·ÖÏí] ÔõÑùÃèÊöSCIÂÛÎÄÖеÄʵÑé·½·¨²¿·Ö ÒÑÓÐ2È˲ÎÓë
|
|
¡°·½·¨¡±²¿·ÖÓ¦°üº¬×ã¹»µÄÐÅÏ¢ÒÔ¹©ÆäËûÑо¿Õß×¼È·ÖØ¸´ÄãÃèÊöµÄÊÔÑé;Èç¹ûÆäÖÐÓйؼüÐÅÏ¢ÒÅ©£¬±ðÈ˾ÍÎÞ·¨ÍêÈ«ÖØ¸´ÄãµÄʵÑéÌõ¼þ;Õâ»áÒýÆð½á¹û²»Ò»Ö£¬ÓпÉÄÜÔì³ÉÎó»á£¬ÉõÖÁ»¹»á±»ÈËÖ¸ÔðÔì¼Ù¡£ËùÒÔ¡°·½·¨¡±²¿·ÖÒªÁ¦ÇóÈ«Ãæ¡£ ¡°·½·¨¡±²¿·ÖÓ¦¸ÃʹÓùýȥʱ̬£¬ÀýÈ磺¡°sections were stained with¡¡± and ¡°data were analyzedusing¡¡±¡£µ«ÊÇÔÚÌἰͼ±íʱӦʹÓÃÏÖÔÚʱ£¬ÀýÈ磺¡°The patients¡¯ clinical characteristics arelisted in Table 1¡±¡£ ´ËÍ⣬µ±ÄãÔÚÐðÊöij¸öÊÂÎïµÄ¶¨Òå»òµ±Ç°¶ÔËü¹«ÈϵĿ´·¨Ê±Ò²ÒªÓÃÏÖÔÚʱ£¬ÀýÈ磺¡°the cells weresubjected to hypoxia, which induces HIF-1expression¡¡±¡£Õâ¾ä»°ÖУ¬ËäȻʵÑ鲿·ÖÊÇÓõĹýȥʱ£¬µ«ÓÉÓÚȱÑõµ¼ÖÂHIF-1ÊÇÒ»¸öÆÕ±éÏÖÏó¶ø²»ÊÇÏÞ¶¨ÓÚ±¾ÎĵĽá¹û£¬Òò´ËºóÃæ°ë¾äÓ¦¸ÃÓÃÏÖÔÚʱ¡£ ÒªÁгöËùÓÐÊÔ¼ÁµÄ¹©Ó¦ÉÌÒÔ¼°É豸µÄ³§¼Ò;ÓÐµÄÆÚ¿¯»¹ÒªÇóÁгöÆäµØÖ·£¬¼´¹ú¼Ò¡¢ÖÝ(ÈçΪÃÀ¹ú)ºÍÊС£ÐðÊöÉ豸¡¢ÊÔ¼ÁºÐ¡¢ÊÔ¼ÁµÄʱºòÓ¦¸ÃÓó£ÓÃÊõÓïÀ´¾ßÌåÃèÊö£¬²»ÒªÖ»Óó§É̵ÄרÃÅÊõÓï»òֻдÐͺš£±ÈÈ磬²»ÒªÐ´³É¡°Absorbance in each well was measured at a wavelength of 492 nm usinga BeckmanCoulter AD 340C¡±£¬ÒòΪ´ó¶àÊýÈ˶¼¶ÔBeckman Coulter AD340CûÓÐÈκθÅÄî;Ó¦¸Ãд³É£º¡°Absorbance in each well was measured at a wavelengthof 492 nm using a multi-well plate reader (AD 340C, BeckmanCoulter)¡±£¬»òÕß¡°Absorbance in each well was measured at a wavelength of492 nm using an AD 340C multi-well plate reader (Beckman Coulter Inc,Fullerton, CA, USA)¡±¡£ ¡°·½·¨¡±²¿·ÖÖ»ÄܰüÀ¨×îÖյóö½á¹ûµÄʵÑé·½·¨¡£Èç¹ûij¸öÊÔÑéʧ°ÜÁË»òÕßûÄÜÌṩÄãÐèÒªµÄ½á¹û£¬¶øÇÒÄãÒѾ¾ö¶¨ÂÛÎÄÖв»ÌáÕâЩ½á¹û£¬ÄÇôҲûÓбØÒªÈ¥Ì¸ÆäÊÔÑé·½·¨¡£ÓÃÊʵ±µÄ×Ó±êÌâ°Ñ¸÷ÖÖ²»Í¬Ä¿µÄµÄ²ÄÁϺͷ½·¨·ÖÀà¡£Èç¹ûÆÚ¿¯ÉèÓС°²¹³ä·½·¨¡±(SupplementaryMethods)²¿·Ö£¬Ôò¿ÉÓÃÕⲿ·ÖÀ´ÏêÊöϸ½Ú£¬²¢ÈÃÖ½Ãæ¿¯³öµÄ¡°·½·¨¡±²¿·Ö±£³Ö¼ò¶Ì¡£Ð·½·¨Ó¦¸ÃÏêϸÐðÊöÒÔ±ãËûÈËÖØ¸´;±ê×¼ºÍ³£Óü¼ÊõÖ»ÐëÒýÓÃÎÄÏ×¼´¿É£¬µ«Èç¹ûÄãµÄ·½·¨ºÍÎÄÏ×ÓвîÒìÖ®´¦Ó¦Óè˵Ã÷¡£ÓÈÆäÒª±£Ö¤ËùÓе¥Î»¶¼ÕýÈ·£¬ÊµÑéÌõ¼þ(Èçʱ¼ä¡¢Î¶È)¶¼Çå³þ¡£×îºó£¬Èç¹û¿ªÕ¹ÁËͳ¼ÆÑ§·ÖÎöÀ´ÆÀ¼ÛÑо¿½á¹ûµÄÒâÒ壬ÔòÓ¦ÔÚ¡°·½·¨¡±µÄ×îºóÒ»¶ÎÐðÊöÄãµÄͳ¼ÆÑ§·½·¨£¬°üÀ¨ËùÑ¡ÔñµÄÏÔÖøÐÔãÐÖµ¡£ ʵÀý ¡°¡homogenates were spun at 10,000 ¡Á g and 4 ¡ãC for 12 min¡±Õâ¾ä»°°üº¬ÁËʱ¼ä¡¢Î¶ÈÁ½¸öÖØÒªÏ¸½Ú¡£¶ø£º¡°¡homogenates were spun at 10,000 ¡Á g¡± ºÍ¸ü¼ò»¯µÄ ¡°homogenates were centrifuged¡± Á½¸ö¾ä×ÓÖоÍÒÅ©ÕâЩϸ½Ú¡£ÕâЩϸ½ÚÓпÉÄܶԵõ½ÄãµÄ½á¹ûºÜÖØÒª£¬ËùÒÔÓ¦¸Ã¸ø³ö¡£ ͬÀí£¬²»ÒªÖ»Ð´£º¡°Then, 10 ¦Ìl of a propidium iodide solution was added to thecells¡±£¬ÒòΪÕâ¶Ô¶ÁÕߺÁÎÞÒâÒ壬³ý·ÇËûÃÇÖªµÀ¸ÃÈÜÒºµÄŨ¶È¡£Ó¦¸Ãд³É£º¡°Propidium iodide was added to thewells to a final concentration of 0.5 ¦Ìg/ml¡±¡£ ת×Ô¿ÆÑ§Íø£ºhttp://bbs.sciencenet.cn/thread-2162554-1-1.html |
» ÊÕ¼±¾ÌûµÄÌÔÌûר¼ÍƼö
µç»¯Ñ§´ß»¯ |
» ²ÂÄãϲ»¶
295Çóµ÷¼Á
ÒѾÓÐ3È˻ظ´
Ò»Ö¾Ô¸211ԺУ 344·Ö ¶«±±Å©Òµ´óѧÉúÎïѧѧ˶£¬Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´
085602 307·Ö Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
322Çóµ÷¼Á
ÒѾÓÐ3È˻ظ´
¸ßθßѹ·´Ó¦ÇóÖú
ÒѾÓÐ3È˻ظ´
Ò»Ö¾Ô¸ÉϺ£Àí¹¤ÄÜÔ´¶¯Á¦£¨085800£©310·ÖÇóµ÷¼Á
ÒѾÓÐ4È˻ظ´
ʳƷ¹¤³Ìר˶һ־ԸÖк£Ñó309Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
285Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
289Çóµ÷¼Á
ÒѾÓÐ7È˻ظ´
Çóµ÷¼Á
ÒѾÓÐ4È˻ظ´
» ±¾Ö÷ÌâÏà¹ØÉ̼ÒÍÆ¼ö: (ÎÒÒ²ÒªÔÚÕâÀïÍÆ¹ã)
» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:
¹ØÓÚSCIÂÛÎÄÖÐÉãÊ϶ȵÄ×îбíʾ·½·¨
ÒѾÓÐ3È˻ظ´
SCIÎÄÕ ʹÓÃ×Ô¼ºÎÄÕµľä×ÓËã³Ï®Âð
ÒѾÓÐ6È˻ظ´
sciÂÛÎÄÔõôд£¿
ÒѾÓÐ10È˻ظ´
ÂÛÎÄ˼·£¬Ïë·¢SCI£¬Çë¸÷λ´óÉñ¿´¿´
ÒѾÓÐ10È˻ظ´
×Ô¼ºÐ´µÄÎÄÕÂÖеÄʵÑé·½·¨¿ÉÒÔÕÕ³Â𣿣¿£¿
ÒѾÓÐ5È˻ظ´
ʵÑé·½°¸Ôõôд
ÒѾÓÐ5È˻ظ´
×Ô¼ºÐ´µÄsci·¢±íÁË£¬»¹ÄÜÓÃÀïÃæµÄÊý¾Ý·¢ÖÐÎÄÂð£¿
ÒѾÓÐ12È˻ظ´
°´ÕÕ±ðÈ˵ķ½·¨×öµÄʵÑ飬µ«ÊµÑé½á¹û²»Ì«Ò»Ñù£¬»¹Äܲ»ÄÜ·¢±í£¿
ÒѾÓÐ6È˻ظ´
²»ÊǺܺõÄʵÑé½á¹ûÔõôд³öˮƽ¸ßµã£¨¹úÄÚSCIÒÔÉÏ£©µÄÂÛÎİ¡£¿
ÒѾÓÐ17È˻ظ´
½»Á÷£ºÈçºÎÌá¸ßSCIÂÛÎĵÄËûÒý´ÎÊý£¿
ÒѾÓÐ5È˻ظ´
SCIÀïÃæ£¬ÊµÑé²ÄÁÏÊÇʲôƷÖÖ¼¸ºÅµÄ»°Ôõôд°¡
ÒѾÓÐ4È˻ظ´
Ó¢ÎÄÎÄÕÂÈçºÎÒýÓÃÖÐÎÄÎÄÕ£¿
ÒѾÓÐ11È˻ظ´
Ó¢ÎÄSCIÂÛÎÄд×÷¡¢Í¶¸å¹ý³ÌÖеÄ×¢ÒâÊÂÏî
ÒѾÓÐ14È˻ظ´
ʵÑ鲿·ÖµÄ²Î¿¼ÎÄÏ×Ôõôд£¿
ÒѾÓÐ5È˻ظ´
ÇëÎÊÂÛÎÄд×÷ÖеÄʵÑ鲿·Ö¿ÉÒÔÔËÓÃרÀûÖеķ½·¨Âð£¿
ÒѾÓÐ4È˻ظ´
¸öÈ˵ÄÒ»µãSCIд×÷¡¢ÈóÉ«¡¢Í¶¸å֮·
ÒѾÓÐ83È˻ظ´
SCIÖÐÓÐûÓÐÓÃÕý½»ÊÔÑéÉè¼ÆµÄ?
ÒѾÓÐ29È˻ظ´
±ÏÒµÂÛÎĵÄÇ°ÑØ£¬ÊµÑé·½·¨ºÍ×ÛÊöµÄд·¨
ÒѾÓÐ14È˻ظ´

congqin312
°æÖ÷ (ÖªÃû×÷¼Ò)
~~ûÐÄû·ÎµÄС³Ô»õ
- Ó¦Öú: 30 (СѧÉú)
- ¹ó±ö: 0.509
- ½ð±Ò: 24486.9
- É¢½ð: 6365
- ºì»¨: 148
- ɳ·¢: 129
- Ìû×Ó: 9636
- ÔÚÏß: 1365.6Сʱ
- ³æºÅ: 1405025
- ×¢²á: 2011-09-17
- ÐÔ±ð: MM
- רҵ: Óлú¸ß·Ö×Ó¹¦ÄܲÄÁÏ
- ¹ÜϽ: ÊÖ»ú×ÊÔ´

2Â¥2014-11-23 12:05:11
![]() |
3Â¥2014-11-23 12:09:26
|
4Â¥2014-11-24 10:13:33















»Ø¸´´ËÂ¥


