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Cytogenetic Analysis of Asiatic Lily Cultivars and Their Hybrids Using Fluorescence In Situ Hybridization ×÷Õß:Gao, T (Gao, T.)[ 1 ] ; Sun, H (Sun, H.)[ 1 ] ; Fang, L (Fang, L.)[ 1 ] ; Qian, H (Qian, H.)[ 1 ] ; Xin, H (Xin, H.)[ 1 ] ; Shi, J (Shi, J.)[ 1 ] ; Wu, Z (Wu, Z.)[ 1 ] ; Xi, M (Xi, M.)[ 1 ] ±àÕß:VanTuyl, JM III INTERNATIONAL SYMPOSIUM ON THE GENUS LILIUM ´ÔÊé: Acta Horticulturae ¾í: 1027 Ò³: 177-184 ³ö°æÄê: 2014 »áÒéÃû³Æ »áÒé: 3rd International Symposium on the Genus Lilium »áÒ鵨µã: Zhangzhou, PEOPLES R CHINA »áÒéÈÕÆÚ: APR 01-03, 2014 »áÒéÔÞÖúÉÌ:Int Soc Hort Sci ÕªÒª Two Asiatic lily cultivars, 'Golden Horn', 'Pollyanna', and their six progenies were analyzed using Fluorescence in situ hybridization (FISH) with 45S rDNA as probe. It was shown that five progenies were diploid (2n=2x=24), one progeny was triploid (2n=3x=36), We observed a total of 11 hybridization signals on chromosome No 1, 2, 3, 4, 7 and 11 in 'Golden Horn', 10 hybridization signals occurred on chromosome No 1, 2, 4, 6, 7 and 11 in 'Pollyanna'. 45S rDNA loci were variable on chromosome No 3 and 6 of these two cultivars. Chromosome No 3 in 'Golden Horn' contained two 45S rDNA loci which were observed at the centromere and long arm, while 45S rDNA locus on chromosome No 6 in 'Pollyanna' was on the long arm. In addition, 45S rDNA loci on chromosome No 1 and 7 were located near the centromere, on chromosome No 2 the signals were near the secondary constriction and on chromosome No 4 and 11 were on the long arm. There were either 9 or 11 45S rDNA loci in the five diploid hybrids, and there were 15 loci in the triploid progeny. FISH detection of 45S rDNA clearly showed that all 45S rDNA signals in four progenies (GHP16, GHP142, GHP223, GHP245) were coming from 'Golden Horn' and 'Pollyanna', respectively. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Lilium; hybrids; fluorescence in situ hybridization; 2n gametes KeyWords Plus:SEXUAL POLYPLOIDIZATION; LILIUM; INTROGRESSION; MECHANISMS; PLANTS; GISH ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Xi, M (ͨѶ×÷Õß) ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Jiangsu, Peoples R China. µØÖ·: ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 1 ] Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Jiangsu, Peoples R China µç×ÓÓʼþµØÖ·:ximenglinjfu@126.com ³ö°æÉÌ INT SOC HORTICULTURAL SCIENCE, PO BOX 500, 3001 LEUVEN 1, BELGIUM Àà±ð / ·ÖÀà Ñо¿·½Ïò lant Sciences; AgricultureWeb of Science Àà±ð lant Sciences; HorticultureÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ roceedings PaperÓïÖÖ:English Èë²ØºÅ: WOS:000343865600019 ISBN:978-94-62610-05-7 ISSN: 0567-7572 ÆäËûÐÅÏ¢ IDS ºÅ: BB5GX Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 20 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 |
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lant Sciences; Agriculture