24小时热门版块排行榜

>论坛更新日志 (4388)
>考研 (1765)
>导师招生 (883)
>休闲灌水 (319)
>虫友互识 (317)
>文献求助 (160)
>考博 (153)
>论文投稿 (122)
>硕博家园 (102)
>公派出国 (74)
>招聘信息布告栏 (49)
>博后之家 (45)
>教师之家 (35)
>基金申请 (34)
>找工作 (29)
>论文道贺祈福 (19)

返回列表

当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖

宋伟同学

铜虫 (著名写手)

应助: 14 (小学生)
金币: 1020.5
散金: 80
红花: 10
帖子: 1586
在线: 129.5小时
虫号: 2494321
注册: 2013-06-03
专业: 神经生物学

[交流] 请问LexA::GAD， repo-LexA::GAD，lexAop-rCD2::GFP，UAS-mCD8::RFP，这都是啥。已有1人参与

原文如下：
To address this question, we repeated clonal analysis with
dual-expression-controlMARCMthatpermitsdifferentiallabel-
ing of distinct cells in a given MARCM clone owing to indepen-
dent controls over distinct reporter genes by GAL4 versus
LexA::GAD, two GAL80-suppressible binary transcriptional ac-
tivators (Lai and Lee, 2006). We used repo-LexA::GAD plus the
ensheathing glial GAL4 driver, NP6520, in dual-expression-
control MARCM, to label GAL80-minus ensheathing glia with
bothlexAop-rCD2::GFPandUAS-mCD8::RFPwhilemarkingthe
astrocyte-like glia of the same genotype with lexAop-rCD2::GFP
only. If a neuropil glial cluster consisted of only ensheathing or
astrocyte-likeglia,onewouldexpectexpressionofmCD8::RFPin
all or none of the cells in the cluster. In contrast, if ensheathing
and astrocyte-like glia derived from common progenitors, one
would detect mCD8::RFP in a subset of rCD2::GFP-marked cells
in a single cluster. Fifteen clusters of neuropil glia were obtained
following induction of somatic recombination at the newly
hatched larval stage. Among them, 12 clusters were found to
purely consist of either ensheathing glia or astrocyte-like glia, as
evidenced by the expression of mCD8::RFP in all or none of the
cellsinagivencluster(Fig.7E–H).Intheotherthreeclustersthat
containedbothmCD8::RFP-positiveand-negativecells,nomat-
ter whether ensheathing or astrocyte-like glia predominated, the
minority was overwhelmed and composed of only one or two
cells per cluster despite the cluster size being roughly ?8 cells.
Sincealowlevelofmitoticrecombinationcouldoccurevenwith-
out any heat-shock induction (Fig. 4R), it is possible that the
presence of one or two heterotypic cells in the otherwise homo-
geneous population may occur as a consequence of multiple
events of mitotic recombination. Given that ensheathing and
astrocyte-like glia rarely coexist in a single cluster, the two sub-
types of neuropil glia differ not only in morphology and marker
expression but also with different origins of development.

回复此楼