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北京石油化工学院2026年研究生招生接收调剂公告
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宋伟同学

铜虫 (著名写手)

[交流] 请问LexA::GAD, repo-LexA::GAD,lexAop-rCD2::GFP,UAS-mCD8::RFP,这都是啥。 已有1人参与

原文如下:
To address this question, we repeated clonal analysis with
dual-expression-controlMARCMthatpermitsdifferentiallabel-
ing of distinct cells in a given MARCM clone owing to indepen-
dent controls over distinct reporter genes by GAL4 versus
LexA::GAD, two GAL80-suppressible binary transcriptional ac-
tivators (Lai and Lee, 2006). We used repo-LexA::GAD plus the
ensheathing glial GAL4 driver, NP6520, in dual-expression-
control MARCM, to label GAL80-minus ensheathing glia with
bothlexAop-rCD2::GFPandUAS-mCD8::RFPwhilemarkingthe
astrocyte-like glia of the same genotype with lexAop-rCD2::GFP
only. If a neuropil glial cluster consisted of only ensheathing or
astrocyte-likeglia,onewouldexpectexpressionofmCD8::RFPin
all or none of the cells in the cluster. In contrast, if ensheathing
and astrocyte-like glia derived from common progenitors, one
would detect mCD8::RFP in a subset of rCD2::GFP-marked cells
in a single cluster. Fifteen clusters of neuropil glia were obtained
following induction of somatic recombination at the newly
hatched larval stage. Among them, 12 clusters were found to
purely consist of either ensheathing glia or astrocyte-like glia, as
evidenced by the expression of mCD8::RFP in all or none of the
cellsinagivencluster(Fig.7E–H).Intheotherthreeclustersthat
containedbothmCD8::RFP-positiveand-negativecells,nomat-
ter whether ensheathing or astrocyte-like glia predominated, the
minority was overwhelmed and composed of only one or two
cells per cluster despite the cluster size being roughly ?8 cells.
Sincealowlevelofmitoticrecombinationcouldoccurevenwith-
out any heat-shock induction (Fig. 4R), it is possible that the
presence of one or two heterotypic cells in the otherwise homo-
geneous population may occur as a consequence of multiple
events of mitotic recombination. Given that ensheathing and
astrocyte-like glia rarely coexist in a single cluster, the two sub-
types of neuropil glia differ not only in morphology and marker
expression but also with different origins of development.
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宋伟同学

铜虫 (著名写手)

引用回帖:
2楼: Originally posted by liuderong at 2014-11-14 16:28:01
“::”前面表示启动子名称,后面表示基因名称。

谢谢大侠,我要了解这方面知识,求推荐教材。谢谢。
3楼2014-11-15 12:29:45
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liuderong

铁杆木虫 (正式写手)


小木虫: 金币+0.5, 给个红包,谢谢回帖
“::”前面表示启动子名称,后面表示基因名称。
2楼2014-11-14 16:28:01
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liuderong

铁杆木虫 (正式写手)


小木虫: 金币+0.5, 给个红包,谢谢回帖
这个符号仅仅是一种约定,貌似没有什么值得特别学习的!你想了解哪方面的信息的?

[ 发自手机版 http://muchong.com/3g ]
4楼2014-11-15 19:52:13
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宋伟同学

铜虫 (著名写手)

引用回帖:
4楼: Originally posted by liuderong at 2014-11-15 19:52:13
这个符号仅仅是一种约定,貌似没有什么值得特别学习的!你想了解哪方面的信息的?

先谢谢大侠回复我。我就是头一次看到这些东西吗,也不知道是啥,我想知道这些东西是怎么来的,怎么用的,有什么特点,使用策略,注意事项。我也用百度查过,都是些说的很浅的东西。英文的我又很着急,非常感谢。
5楼2014-11-16 12:21:36
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