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The conclusion that TNF¦Áis a trigger of spontaneous pain, also be proved with minocycline preoperative in mSNA rats. Because the TNF¦Áexpression was suppressed in mSNA rats with minocycline preoperative. As we known, minocycline is a strong anti-inflammatory drug. Minocycline can significantly suppress macrophages recruitment, microglial activation [39], inflammatory cytokines releasing. Thus it can suppress TNF¦Áexpressing in the spinal dorsal horn of mSNA rats in our studying. And the SFL behavior was alleviated too. Our result is also consistent with Ik-Hyun Cho report that the SFL behavior is suppressed with minocycline pre-surgery in the inflammatory pain rats [14].

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The conclusion that TNF¦Áis a trigger of spontaneous pain, also be proved with minocycline preoperative in mSNA rats. Because the TNF¦Áexpression was suppressed in mSNA rats with minocycline preoperative. As we all known, minocycline is a kind of drug with strong anti-inflammatory . Minocycline can deeply suppress  recruitment of macrophages, microglial activation [39], inflammatory cytokines releasing. Thus it can suppress TNF¦Áexpressing in the spinal dorsal horn of mSNA rats in our studies. And the SFL behavior was alleviated too. Our result is also consistent with Ik-Hyun Cho report that the SFL behavior is suppressed with minocycline pre-surgery in the inflammatory pain rats [14].
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