| 查看: 733 | 回复: 4 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[求助]
一篇文献中的噬菌体核酸提取，求大神讲解啊 已有1人参与
|
||
|
本人小小研一学生一枚，求助求助。 2.4 Isolation of Phage Nucleic Acids 1)The phage filtrate having 8% polyethylene glycol (PEG) and 4% NaCl was centrifuged at 26,000 rpm for 4 h. 2) Dissolve the pellet in 100 μL autoclaved distilled water and sonicate it for 3 min. Centrifuge it again for 5 min at 14,000 rpm. 3)One microliter of DNaseI was added and incubated at 37°C for 30 min. After incubation,4 μL of 2.5 X SDS-EDTA dye (0.4% SDS, 30 mM EDTA, 0.25% bromophenol blue, 20% sucrose) was added to the tubes and kept at 80°C in Heat Block for 10 min. After heat treatment, the whole lysate was loaded onto 0.7% TAE agarose gel (Tris base, boric acid, EDTA, pH 8.3) containing 0.5 μg/mL of ethidium bromide. 4)The gel was run at a voltage of 90 V and the bands were visualized using a UV transilluminater. |
回复此楼» 猜你喜欢
Wnt-C59：PORCN靶向化学探针，Wnt信号通路阻断机制与科研应用全解析
已经有0人回复
-
Fexaramine：肠道限制性FXR化学探针，结构特征与信号通路研究全解析
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有134人回复
-
NVS-STG2：靶向STING跨膜域的分子胶类激动剂，结构特征与寡聚化机制研究全解析
已经有0人回复
-
DOTA-GPC3肽（CAS 3066439-94-6）：双功能分子探针前体的结构特征与标记应用解析
已经有0人回复
-
科研用 TLR7/8 双激动剂怎么选？现货与质控要点
已经有0人回复
-
SEL120-34A HCl：CDK8/CDK19高选择性激酶抑制剂
已经有0人回复
-
氢氧化钾活化操作
已经有18人回复
-
散金
已经有68人回复
-
生物质超级电容器
已经有0人回复
5楼2014-11-20 10:00:32
2楼2014-11-04 17:56:26
3楼2014-11-08 14:13:19
4楼2014-11-20 09:47:49
投票:
10