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纪念SCI诞生50周年的百大高引论文榜单
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为了纪念科学引文索引（SCI）诞生五十周年，Nature委托汤森路透列出了数据库中的百大高引论文。荣登榜首的那篇文章你一定猜不到。 据介绍，这份百强名单的入围资格是引用次数超过12,119。令人意外的是，许多特别知名的论文并未入榜，比如DNA双螺旋结构的确定。而1951年发表的一种蛋白定量方法，狂揽三十多万次引用，成为了最大的赢家。该文章的作者，美国生化学家Oliver Lowry也对此感到困惑。 " Rank Title Times cited 1 Protein measurement with the folin phenol reagent. 305148 2 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 213005 3 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. 155530 4 DNA sequencing with chain-terminating inhibitors. 65335 5 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. 60397 6 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 53349 7 Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density. 46702 8 Density-functional thermochemistry. III. The role of exact exchange. 46145 9 A simple method for the isolation and purification of total lipides from animal tissues. 45131 10 Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. 40289 11 Nonparametric estimation from incomplete observations. 38600 12 Basic local alignment search tool. 38380 13 A short history of SHELX. 37978 14 Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. 36410 15 A revised medium for rapid growth and bio assays with tobacco tissue cultures. 36132 16 Generalized gradient approximation made simple. 35405 17 """Mini-mental state"": A practical method for grading cognitive state of patients for clinician." 34532 18 A rapid method of total lipid extraction and purification. 32131 19 Detection of specific sequences among DNA fragments separated by gel-electrophoresis. 31904 20 The neighbor-joining method: A new method for reconstructing phylogenetic trees. 30176 21 Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. 28870 22 Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides. 28658 23 Processing of X-ray diffraction data collected in oscillation mode. 28647 24 Regression models and life-tables. 28439 25 Density-functional exchange-energy approximation with correct asymptotic-behavior. 26475 26 Colorimetric method for determination of sugars and related substances. 25735 27 Use of lead citrate at high pH as an electron-opaque stain in electron microscopy. 24449 28 The CLUSTAL_X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. 24098 29 Statistical methods for assessing agreement between two methods of clinical measurement. 23826 30 Reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. 23642 31 Isolation of biologically-active ribonucleic-acid from sources enriched in ribonuclease. 23435 32 The attractions of proteins for small molecules and ions. 23421 33 The moderator–mediator variable distinction in social psychological-research — conceptual, strategic, and statistical considerations. 23356 34 Self-consistent equations including exchange and correlation effects. 23059 35 Rapid colorimetric assay for cellular growth and survival — application to proliferation and cyto-toxicity assays. 23011 36 Helical microtubules of graphitic carbon. 22899 37 The colorimetric determination of phosphorus. 22690 38 Disc electrophoresis — II. Method and application to human serum proteins. 22074 39 Inhomogeneous electron gas. 21931 40 A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. 21446 41 Confidence limits on phylogenies: an approach using the bootstrap 21373 42 A new generation of Ca2+ indicators with greatly improved fluorescence properties 19561 43 Efficient iterative schemes for ab initio total-energy calculations using a plane-wave basis set. 18856 44 High-resolution 2-dimensional electrophoresis of proteins. 18489 45 MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. 18286 46 Fuzzy sets. 18203 47 Phase annealing in SHELX-90: direct methods for larger structures. 17728 48 Clinical diagnosis of Alzheimer’s disease: Report of the NINCDS-ADRDA Work Group under the auspices of Department of Health and Human Services Task Force on Alzheimer’s Disease. 17220 49 Special points for Brillouin-zone integrations. 17087 50 Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. 17067 51 The CES-D scale: a self-report depression scale for research in the general population. 17055 52 Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. 17025 53 A rating scale for depression. 16734 54 An inventory for measuring depression. 16264 55 A simple method for displaying the hydropathic character of a protein. 16059 56 Determination of serum proteins by means of the biuret reaction. 16009 57 Maximum likelihood from incomplete data via EM algorithm. 15993 58 Equation of state calculations by fast computing machines. 15902 59 Controlling the false discovery rate: a practical and powerful approach to multiple testing. 15898 60 Measurement of protein using bicinchoninic acid. 15802 61 The assessment and analysis of handedness: the Edinburgh inventory. 15517 62 Estimation of concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. 15469 63 Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. 15160 64 Multiple range and multiple F tests. 15047 65 Electric field effect in atomically thin carbon films. 15022 66 Tissue sulfhydryl groups. 15019 67 Isolation of mononuclear cells and granulocytes from human blood. 14934 68 The measurement of observer agreement for categorical data. 14903 69 Crystallography & NMR system: a new software suite for macromolecular structure determination. 14898 70 Gaussian-basis sets for use in correlated molecular calculations. 1. The atoms boron through neon and hydrogen. 14617 71 PROCHECK: a program to check the stereochemical quality of protein structures. 14462 72 The MOS 36-item short-form health survey (SF-36): I. Conceptual framework and item selection. 14332 73 A new look at statistical-model identification. 14275 74 Improved M13 phage cloning vectors and host strains — nucleotide-sequences of the M13mp18 and pUC19 vectors 14232 75 A comprehensive set of sequence-analysis programs for the vax. 14226 76 MODELTEST: Testing the model of DNA. 14099 77 From ultrasoft pseudopotentials to the projector augmented-wave method. 14049 78 Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. 13881 79 Comparison of simple potential functions for simulating liquid water. 13774 80 The development and use of quantum-mechanical molecular-models. 76. AM1: a new general-purpose quantum-mechanical molecular-model 13718 81 Phosphorus assay in column chromatography. 13523 82 MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. 13496 83 Van der Waals volumes and radii. 13417 84 A new and rapid colorimetric determination of acetylcholinesterase activity. 13332 85 Projector augmented-wave method. 13330 86 Optimization by simulated annealing. 13293 87 Nitric oxide: physiology, pathophysiology, and pharmacology. 13267 88 An algorithm for least-squares estimation of nonlinear parameters. 13258 89 Efficiency of ab initio total energy calculations for metals and semiconductors using a plane-wave basis set. 13084 90 A low-cost, high-efficiency solar-cell based on dye-sensitized colloidal TiO2 films. 12873 91 A low-viscosity epoxy resin embedding medium for electron microscopy. 12807 92 The Protein Data Bank. 12754 93 Accurate and simple analytic representation of the electron-gas correlation-energy 12748 94 Rapid alkaline extraction procedure for screening recombinant plasmid DNA. 12721 95 Improved methods for building protein models in electron-density maps and the location of errors in these models. 12649 96 Accurate spin-dependent electron liquid correlation energies for local spin-density calculations — a critical analysis. 12583 97 Continuous cultures of fused cells secreting antibody of predefined specificity. 12391 98 Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. 12257 99 Adsorption of gases in multimolecular layers. 12252 100 MrBayes 3: Bayesian phylogenetic inference under mixed models 12209 101 Atherosclerosis — an inflammatory disease. |
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