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lixingyu: 金币+5, ★★★很有帮助 2014-11-08 10:42:31
jssxh: LS-EPI+1, 请继续关注本版块 2014-11-12 08:27:35
lixingyu(sunshan4379代发): 金币+5 2014-11-12 10:26:37
Enhancement of Biocontrol Activities and Cyclic Lipopeptides Production by Chemical Mutagenesis of Bacillus subtilis XF-1, a Biocontrol Agent of Plasmodiophora brassicae and Fusarium solani.
作者:Li, Xing-Yu; Yang, Jing-Jing; Mao, Zi-Chao; Ho, Hon-Hing; Wu, Yi-Xing; He, Yue-Qiu
Indian journal of microbiology
卷:54
期:4
页:476-9
DOI:10.1007/s12088-014-0471-y
出版年:2014-Dec (Epub 2014 May 23)
摘要
Bacillus subtilis XF-1 has been used as a biocontrol agent of clubroot disease of crucifers infected by Plasmodiophora brassicae, an obligate pathogen. In order to maximize the growth inhibition of the pathogen, random mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine was applied to strain XF-1. The efficacy of 226 selected mutants was assessed against the growth of an indicator fungal pathogen: Fusarium solani using agar plate assay and the disruptive effects on the resting spores of P. brassicae. Four mutants exhibited inhibition activity significantly higher than the wild type. The cell extracts of these mutants and the XF-1 were subjected to matrix-assisted laser desorption ionization-time of flight mass spectra analysis, and three families of cyclic lipopeptides (CLPs) fengycin, surfactin and iturin were identified from the parental strain and the screened mutants. However, the relative contents and compound diversity changed after mutagenesis, and there was slight variation in the surfactin and fengycin. Notably, only 5 iturin components were discovered from the wild strain XF-1, but 13 were obtained from the mutant strains, and the relative CLPs contents of all mutant strains increased substantially. The results suggested that CLPs might be one of main biocontrol mechanisms of the clubroot disease by XF-1. The 4 mutants are far more effective than the parental strain, and they would be promising biocontrol candidates not only against P. brassicae but probably other plant diseases caused by fungi.
关键词
关键词列表:Cyclic lipopeptides; Fengycin; Iturin; MALDI-TOF-MS; Surfactin
作者信息
地址:National Engineering Center of Application Technologies for Agricultural Diversity, Yunnan Agricultural University (YAU), Kunming, 650201 People's Republic of China.
文献信息
文献类型:Journal Article
语种:English
PubMed ID:25320450
NLM 唯一 ID:0374703
创建日期: 16 Oct 2014 完成日期: 16 Oct 2014 修订日期: 18 Oct 2014
电子出版:23 May 2014
国家/地区: India
ISSN:0046-8991
期刊信息
Impact Factor (影响因子): Journal Citation Reports®
其他信息
记录所有者:NLM
状态 ubMed-not-MEDLINE |
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