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3.2. Large Scale Fermentation of PM0525875-V4 Large scale fermentation was carried out using PM0525875-V4 and fermentation medium ASW-36P(1). The fermentation was carried out till 96 h. The time course of fermentation batch is shown in Table 4 and expressed in graphical form as shown in Figure 2. During fermentation, the pH was dropped with the time and later main- tained to 6.5 till end of the fermentation. The rise in biomass (PCV) was slow initially and was picked up after 24 h. After 48 h, the growth rate was steady and the stationary phase was achieved. Dissolve oxygen (DO)dropped significantly till 24 h and was maintained at around 40% till end of the process. The total sugar consumption was slow at beginning and it was reduced to half of the concentration at 48 h. Based on the bioactivity pattern obtained by the broth dilution method (as mentioned in 2.4), the maximum activity was attended at 72 hand maintained steady till end of the fermentation process. Hence, the fermentation cycle was terminated at 96h. |
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