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xiaowanzi9

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[求助] 求助一句话翻译

Following
the exposure period, the cells were processed, re-plated and
incubated during a 7 day "expression" period, and then
reprocessed and grown for 7 days in hypoxanthine-free medium that
contained 24pM 6-thioguanine. The cultures were then fixed and
the numbers of colonies were counted.

Results: No significant change in the rate of cell survival in
the presence of 6-thioguanine (gene mutation) was observed at any
concentration of the test material that was used, either with or
without metabolic activation.
如愿
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xiaowanzi9: 金币+5, 翻译EPI+1, ★★★★★最佳答案, 谢谢你哦 2014-10-23 10:26:46
经过处理期之后(应该是用某种药物处理培养细胞),对细胞进行处理,重新铺板(就是再将细胞接种在培养皿或板或瓶中),经过7天的“表达”期间培养,再加工处理(所谓再加工显示是指将细胞消化下来,收集细胞以便重新培养),并在含有24 pM的6 - 硫鸟嘌呤但不含黄嘌呤的培养基中生长7天。将培养细胞固定后计数集落数目。

显然这是一个集落形成实验(colony forming assay)或者Clonogenic assay.....
2楼2014-10-23 10:08:37
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