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xiaowanzi9新虫 (正式写手)
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Following the exposure period, the cells were processed, re-plated and incubated during a 7 day "expression" period, and then reprocessed and grown for 7 days in hypoxanthine-free medium that contained 24pM 6-thioguanine. The cultures were then fixed and the numbers of colonies were counted. Results: No significant change in the rate of cell survival in the presence of 6-thioguanine (gene mutation) was observed at any concentration of the test material that was used, either with or without metabolic activation. |
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xiaowanzi9: 金币+5, 翻译EPI+1, ★★★★★最佳答案, 谢谢你哦 2014-10-23 10:26:46
xiaowanzi9: 金币+5, 翻译EPI+1, ★★★★★最佳答案, 谢谢你哦 2014-10-23 10:26:46
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经过处理期之后(应该是用某种药物处理培养细胞),对细胞进行处理,重新铺板(就是再将细胞接种在培养皿或板或瓶中),经过7天的“表达”期间培养,再加工处理(所谓再加工显示是指将细胞消化下来,收集细胞以便重新培养),并在含有24 pM的6 - 硫鸟嘌呤但不含黄嘌呤的培养基中生长7天。将培养细胞固定后计数集落数目。 显然这是一个集落形成实验(colony forming assay)或者Clonogenic assay..... |
2楼2014-10-23 10:08:37










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