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tianlus1632木虫 (正式写手)
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[求助]
【农业类】求大神帮忙翻译一段摘要（中译英），非常急用！先谢谢！
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| 以雅安黄油菜开花后10 d的根、茎、叶、花、种皮等不同组织器官和开花后10 d、20 d、30 d、40 d、50 d、60 d、70 d（收获后10 d）的种皮为研究材料，通过实时荧光定量（qPCR）方法对类黄酮途径相关结构基因和调控因子进行表达分析。研究发现：（1）类黄酮途径基因表达总量在花中最高，在根和茎中较少，但黄、褐籽间无明显差异；在叶和开花后10 d的种皮中，类黄酮途径各基因表达总量较高，且褐籽明显高于黄籽。（2）在雅安黄油菜种皮的整个发育时期，黄籽中类黄酮合成途径各基因表达总量呈下降趋势，前、中期下降较快，后期趋于平稳；褐籽种皮发育的前、中期，类黄酮合成途径各基因的表达量逐渐下降，50 d后（即种子成熟阶段），基因的表达总量迅速升高；20 d、60 d和70 d时，黄、褐籽间类黄酮合成途径各基因的表达量差异明显，且越往后差异越大。（3）种皮发育不同时期，黄、褐籽基因表达量差异主要集中在BraANR（LDOX）和BraANS（BAN）基因；20 d时褐籽BraANR基因为黄籽的38.14倍，60 d和70 d时褐籽BraANS基因分别为黄籽的49.93倍和81.58倍。研究认为，雅安黄油菜黄、褐籽种皮颜色的差异受整个类黄酮合成途径各基因的协同影响，但BraANR（LDOX）和BraANS（BAN）这两个基因在其中起到了十分重要的作用。 |
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tianlus1632
木虫 (正式写手)
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- 专业: 作物种质资源与遗传育种学
2楼2014-10-23 09:28:20
LifePursuit
木虫之王 (文学泰斗)
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
tianlus1632: 金币+100, 翻译EPI+1, ★★★★★最佳答案, 谢谢！翻译的很不错，只是有两个句子的意思和中文有点出入，还是谢谢了！ 2014-10-23 11:50:10
phu_grassman: 金币+2, good job. 2014-10-23 16:58:01
tianlus1632: 金币+100, 翻译EPI+1, ★★★★★最佳答案, 谢谢！翻译的很不错，只是有两个句子的意思和中文有点出入，还是谢谢了！ 2014-10-23 11:50:10
phu_grassman: 金币+2, good job. 2014-10-23 16:58:01
| We conducted expression analysis on the flavonoid synthetic pathway related structural genes and regulation factors by real-time fluorescence quantitative PCR (qPCR) assay based on various tissue organs (e.g., roots, stems, leaves, blossoms and seed coats) of Brassica rapa L. (Ya'an Yellow Rape) 10 d after blossoming, as well as seed coats 10 d, 20 d, 30 d, 40 d, 50 d, 60 d and 70 d after blossoming (10 d after being harvested). Our findings include: (1) The total expression of flavonoid pathway genes is the highest in blossoms while the lowest in roots and stems, and there is no significant expression difference between yellow and brown seeds. Besides, the total expression in seed coats reaches its maximum 10 d after blossoming, and it is significantly higher in brown seeds than in yellow seeds. (2) The expression of flavonoid synthetic pathway related genes in yellow seeds decreases during the whole developmental stages of seed coats, and the decreasing rate is fairly high during the early and middle stages while steady during the late stage. Moreover, the expression in brown seeds decreases steadily during the early and middle stages while increases rapidly from 50 d after blossoming (i.e., seed mature stage). There is a significant expression difference between yellow and brown seeds 20 d, 60 d and 70 d after blossoming, and this difference gets increasing with the increase of time. (3) The expression difference between yellow and brown seeds concentrates on BraANR (LDOX) and BraANS (BAN) genes during the whole developmental stages of seed coats. More concretely, the expression of BraANR (LDOX) gene in brown seeds is 38.14, 49.93 or 81.58 times that in yellow seeds 20 d, 60 d or 70 d after blossoming, respectively. We conclude that, with these observations, there is a synergistic effect among all flavonoid synthetic pathway related genes on the color difference of brown and yellow seeds, while BraANR (LDOX) and BraANS (BAN) genes are the most prominent in this synergistic effect. |
3楼2014-10-23 11:11:36
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