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DOI: 10.1016/j.carbpol.2013.01.034
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Extraction of crude polysaccharides from Gomphidius rutilus and their antioxidant activities in vitro

×÷Õß:Wang, CY (Wang, Chenyu)[ 1 ] ; Zhang, J (Zhang, Jing)[ 1 ] ; Wang, F (Wang, Fei)[ 1 ] ; Wang, ZY (Wang, Zhanyong)[ 1 ]

CARBOHYDRATE POLYMERS

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DOI: 10.1016/j.carbpol.2013.01.034

³ö°æÄê: APR 15 2013

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Response surface methodology was used to optimize the extraction of crude polysaccharides from the fruiting bodies of Gomphidius rutilus. A central composite design was adopted to determine the combination of factors (extraction time, extraction temperature, extraction frequency, and ratio of water to raw material) that resulted in the maximum crude polysaccharide production. Results showed that the optimum extraction conditions were as follows: extraction temperature, 95 degrees C; ratio of water to raw material, 16; extraction time, 2.5 h; and extraction frequency, 4. Under these conditions, the experimental yield of crude polysaccharides was 8.02 +/- 0.15%, which well agreed with the predicted yield. Evaluation of the antioxidant activity in vitro suggested that the crude polysaccharides had high scavenging activity for superoxide anion, hydroxyl, and 1,1-diphenyl-2-picrylhydrazyl radicals. The crude polysaccharides also showed strong reducing power. Thus, they can be used as natural antioxidants in functional foods or medicine. (C) 2013 Elsevier Ltd. All rights reserved.
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×÷Õ߹ؼü´Êolysaccharides; Response surface methodology; Gomphidius rutilus; Antioxidant

KeyWords Plus:RESPONSE-SURFACE METHODOLOGY; OPTIMIZATION; PARAMETERS; ANTITUMOR
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ͨѶ×÷ÕßµØÖ·: Wang, ZY (ͨѶ×÷Õß)
[ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ]         Liaoning Shihua Univ, Sch Environm & Biol Engn, Fushun 113001, Peoples R China.

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[ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ]         [ 1 ] Liaoning Shihua Univ, Sch Environm & Biol Engn, Fushun 113001, Peoples R China

µç×ÓÓʼþµØÖ·:wangzy125@gmail.com
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Science Technology Foundation of Fushun city        
FSKJHT201135
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ELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
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Ñо¿·½Ïò:Chemistry; Polymer Science

Web of Science Àà±ð:Chemistry, Applied; Chemistry, Organic; Polymer Science
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ÎÄÏ×ÀàÐÍ:Article

ÓïÖÖ:English

Èë²ØºÅ: WOS:000317888500064

ISSN: 0144-8617
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Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 25

Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 2
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