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| DOI: 10.1016/j.carbpol.2013.08.073 |
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Extraction of polysaccharides from Phellinus nigricans mycelia and their antioxidant activities in vitro ×÷Õß:Wang, ZY (Wang, Zhanyong)[ 1 ] ; Wang, CY (Wang, Chenyu)[ 1 ] ; Quan, Y (Quan, Yue)[ 1 ] CARBOHYDRATE POLYMERS ¾í: 99 Ò³: 110-115 DOI: 10.1016/j.carbpol.2013.08.073 ³ö°æÄê: JAN 2 2014 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª In this study, response surface methodology was employed to optimize the extraction of polysaccharides from Phellinus nigricans mycelia. A central composite design was adopted to determine optimum parameters (extraction time, extraction temperature, extraction frequency, and ratio of water to raw material) that could yield a maximum polysaccharide. Results revealed the following optimum extraction conditions: extraction time, 2.8 h; ratio of water to raw material, 28; extraction frequency, 5; and extraction temperature, 95 degrees C. Under optimized conditions, the experimental yield of P. nigricans mycelia polysaccharides was 15.33 +/- 0.21%, which is consistent with the predicted yield. The antioxidant activity assay in vitro showed that the polysaccharides exhibited a high scavenging activity against superoxide anion, hydroxyl, and 1,1-diphenyl-2-picrylhydrazyl radicals. These polysaccharides also exhibited a strong reducing power. Thus, these polysaccharides can be used as natural antioxidants in functional foods or medicine. (C) 2013 Elsevier Ltd. All rights reserved. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê olysaccharides; Response surface methodology; Phellinus nigricans; Mycelia; AntioxidantKeyWords Plus:RESPONSE-SURFACE METHODOLOGY; ASSISTED EXTRACTION; OPTIMIZATION; ANTITUMOR; IGNIARIUS; IMMUNITY ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Wang, ZY (ͨѶ×÷Õß) [ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ] Liaoning Shihua Univ, Sch Environm & Biol Engn, Fushun 113001, Peoples R China. µØÖ·: [ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ] [ 1 ] Liaoning Shihua Univ, Sch Environm & Biol Engn, Fushun 113001, Peoples R China µç×ÓÓʼþµØÖ·:wangzy125@lshu.edu.cn »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ Science Technology Foundation of Fushun city FSKJHT201135 ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ ELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND Àà±ð / ·ÖÀà Ñо¿·½Ïò:Chemistry; Polymer Science Web of Science Àà±ð:Chemistry, Applied; Chemistry, Organic; Polymer Science ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000329256600014 ISSN: 0144-8617 µç×Ó ISSN: 1879-1344 ÆÚ¿¯ÐÅÏ¢ Ŀ¼£º Current Contents Connect® Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: 283OM Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 16 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 4 |

2Â¥2014-09-23 19:34:35
muse
¾èÖú¹ó±ö (ÖªÃû×÷¼Ò)
- LS-EPI: 147
- Ó¦Öú: 314 (´óѧÉú)
- ¹ó±ö: 0.549
- ½ð±Ò: 20674
- É¢½ð: 2783
- ºì»¨: 81
- ɳ·¢: 156
- Ìû×Ó: 6098
- ÔÚÏß: 1468.2Сʱ
- ³æºÅ: 1207111
- ×¢²á: 2011-02-19
- רҵ: ±£ÏÕѧ

3Â¥2014-09-23 19:34:48













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olysaccharides; Response surface methodology; Phellinus nigricans; Mycelia; Antioxidant