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xgxofdream银虫 (著名写手)
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请教:interspecific backcross vs Mouse genetics mapping
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下面这段文字摘自1990年的一篇关于mouse genetics mapping的综述.这段文字讲了利用"种间回交"来mapping,但是我没看懂它的原理.请遗传学的朋友帮我解释下利用种间回交来做图的原理? Thus, in 1972 the linkage map appeared for the first time with the linkage groups assigned to chromosomes (GREEN 1972)M. apping then entered a new phase involving the precise location of genes, both in terms of their recombination with other genes and in their physical location with respect to chromosome Gbands. A recent advance in methodology has again involved the use of wild mice. GU~NET anhdis colleagues (AVNERet al. 1988) showed that, if subspecies or closely related species of mice are compared, restriction fragment length variants (RFLVs) can be found for nearly all probes with the use of only one or two retriction enzymes. Thus, if laboratory mice are crossed with a wild species, usually Mus spretus, and the F1 female is backcrossed to the laboratory strain (in a so-called interspecific backcross), all genes or other DNA markers can be mapped by their RFLVs. If DNA from individual backcross animals is stored, successive markers can be mapped, so that a panel of DNA from backcross animals becomes a resource which yields more detailed information as time progresses. DNA from animals with recombination within a certain interval can be used for further and finer mapping within that interval, thus enabling “homing in” on a region of interest as, for instance, in attempts to clone a gene. |
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2楼2008-04-07 19:31:39
xgxofdream
银虫 (著名写手)
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3楼2008-04-07 22:47:58
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