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小木虫论坛-学术科研互动平台 » 化学化工区 » 分析 » [求助]Extinction Coefficient of Maleimide
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linger2007212

[交流] [求助]Extinction Coefficient of Maleimide

What is the extinction coefficient of maleimide? How could I find it? Thanks a lot!
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1楼 2008-04-01 05:19:47
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dryfish

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it can be obtained by yourself.
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2楼2008-04-01 08:36:05
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linger2007212

But I don't have maleimide. I wanna determine how active the maleimide group in Maleimdie-PEG.
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3楼2008-04-01 09:49:27
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dryfish

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dnp(金币+1,VIP+0):Thank you for your discussion. Can you back again to help linger2007212 to answer her questions?
how can u determine the activity of the maleimide group in Maleimdie-PEG by its extinction coefficient.
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4楼2008-04-01 10:15:56
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linger2007212

I know the concentration of Maleimide-PEG by weighting its mass and calculation. I wanna measure the UV-Vis absorbance of maleimide by Absorbance=Extinction Coefficient X Active Maleimide Concentration X Path Width  If all the parameters are known except Active Maleimide Concentration, I can calculate it. What I wanna know is that how many maleimide groups are there per PEG.
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5楼2008-04-01 10:39:46
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dryfish

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However, i think your method maybe not works well, because the absorption profile of maleimide also depends on the groups linked to it (here is PEG).
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6楼2008-04-02 08:39:21
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