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wenkoll

铜虫 (初入文坛)

[求助] 跪求Griess reagent (modified)的使用已有1人参与

大家好,小弟在做一氧化氮自由基清除实验,老板买了Sigma 的Griess reagent (modified),以前没用过,查了很多文献也没找到用这个做实验的,有哪位哥哥姐姐用过Griess reagent (modified),帮帮忙,分享一下你的操作方法,特别是Griess reagent的浓度以及用什么溶液配置。非常感谢
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shuang0220

金虫 (正式写手)

好样的

Nitrite (NO2–) detection by Griess Reagent. 50 μL of the sample was taken and
transferred into a cuvette and combined with 50 μL of Griess Reagent and 0.9 mL of Milli-Q
water. The resulting sample was mixed thoroughly. The mixture was incubated at room
temperature in the dark for 0.5 h prior to UV-Vis testing. Solutions of NaNO2 with known
concentrations (in 10 mM H2SO4) were used as calibration standards, with the absorbance at
525 nm used to plot the calibration curves. Upper LOD of UV-Vis used in this study refers to
the absorbance at 525 nm obtained from 200 μM NaNO2. A dilution factor was applied to
measure nitrite concentration in plasma-activated water (PAW)
耐得住寂寞,方能提升自我
4楼2021-01-24 15:47:09
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jnu2012

木虫 (小有名气)

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
辣笔v小新: 金币+1, 鼓励应助交流 2014-06-28 02:16:02
wenkoll: 金币+10, ★★★很有帮助, 非常感谢 2014-09-10 11:48:33
Griess试剂盒的使用说明书
http://pan.baidu.com/s/1i35O58p
2楼2014-06-28 00:23:15
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张天成

新虫 (初入文坛)

楼主,会了吗?本人现在也遇到同样的问题,还请讲解一下。
3楼2018-12-27 17:23:34
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