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現代科學研究提供了提取和檢測杜仲葉片化合物的技术, 所采用的空心分子印迹聚合物（h-mip1）擁有良好的高性能印記效果。五種成分如绿原酸，咖啡酸，香草酸，原儿茶酸和没食子酸已經確定。詳細比較化合物的含量和比例，表明绿原酸高於其它化合物。作为一個研究的焦點，采用超声技術和(HPLC)方法進一步考慮了绿原酸的提取條件,最佳工藝條件為:甲醇水（70%）；溶剂比为20／1（V/W）；提取时间3x30分鍾。 Modern scientific research provide one of the technique for the extraction and determination of some compounds from EUO leaves, it is using a hollow imprinted polymer (H-MIP1) was observed has a good imprint effect and a high capability. The confirmation of five components such as chlorogenic acid (CGA), caffeic acid (CA), vanillic acid (VA), protocatechuic acid (PCA) and gallic acid (GA). The content and proportion of those compounds were compared in details, indicating that the CGA is high than other compounds. As a studies focuses, it goes further to considered of CGA on extraction conditions using ultrasonic technique and (HPLC) method. The optimum extraction conditions: aqueous methanol (70%); solvent/sample ratio 20/1 (v/w) and extraction time 3x30 min. |
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【答案】应助回帖
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RXMCDM: 金币+1, 多谢应助！ 2014-06-18 15:14:36
YauYau: 金币+10, 翻译EPI+1, ★★★很有帮助, Thanks! 2014-06-19 05:38:14
RXMCDM: 金币+1, 多谢应助！ 2014-06-18 15:14:36
YauYau: 金币+10, 翻译EPI+1, ★★★很有帮助, Thanks! 2014-06-19 05:38:14
| Modern scientific research has developed the technique for the extraction and determination of some compounds in EUO leaves. this technique of using a hollow imprinted polymer (H-MIP1) is proved to have a good imprint effect and a high capability. The confirmation of five components include chlorogenic acid (CGA), caffeic acid (CA), vanillic acid (VA), protocatechuic acid (PCA) and gallic acid (GA) was achieved. The content and proportion of these compounds were compared in details,indicating that the CGA is higher than others. As a research focus,we further examine the extraction conditions for CGA using ultrasonic technique and HPLC method. the results shows that The optimum extraction condition is : extraction solution, aqueous methanol (70%); solvent/sample ratio 20/1 (v/w) ; extraction time 3x30 min. |
2楼2014-06-18 14:25:08
