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zhhuijun木虫 (正式写手)
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[求助]
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作者: H.J. Zhang, P. Gao1, X.Z. Wang1 and F.S. Luan 文题: An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.) 期刊名,年份,卷(期),起止页码: Genetics and Molecular Research，第13卷,第1期，54-63页，2014 收录信息: 求助 SCI 收录信息 |
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zhhuijun(sunshan4379代发): 金币+5, 感谢应助！ 2014-07-19 17:34:01
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zhhuijun(sunshan4379代发): 金币+5, 感谢应助！ 2014-07-19 17:34:01
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An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.) 作者:Zhang, HJ (Zhang, H. J.)[ 1,2 ] ; Gao, P (Gao, P.)[ 1 ] ; Wang, XZ (Wang, X. Z.)[ 1 ] ; Luan, FS (Luan, F. S.)[ 1 ] GENETICS AND MOLECULAR RESEARCH 卷: 13 期: 1 页: 54-63 DOI: 10.4238/2014.January.8.4 出版年: 2014 查看期刊信息 摘要 An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation, using cotyledon node zone-stem connection region of melon, has been developed. The new Agrobacterium-mediated transformation methodology, independent of organ culture, used the entire germinated seed as explants. The transformation system was maximized to maintain the integrity of melon itself, thus avoiding the limitations of traditional tissue culture methods. The transformation was carried out under a non-sterile environment. The incorporation of a selectable marker (neomycin phosphotransferase II) into the genome of transgenic plants was confirmed by PCR and Southern blot analyses. The transformation frequency based on the PCR was 13%. Transgenic melon plants were usually detected by PCR in less than 1 month after Agrobacterium inoculation, and seeds could be harvested in 3 months. The growth characteristics and morphology of the transgenic plants were identical to the untransformed wild-type plants. This method would be beneficial for facilitating the characteristics of gene functions and for boosting the manipulation of melon transformation for commercial purposes. 关键词 作者关键词:Transformation method; Agrobacterium tumefaciens; Melon; Adventitious shoots induction KeyWords Plus:ACC OXIDASE GENE; PLANT-REGENERATION; SOMATIC EMBRYOGENESIS; CANTALOUPE; DIVERSITY; CULTIVARS 作者信息 通讯作者地址: Luan, FS (通讯作者) Northeast Agr Univ, Coll Hort, Harbin, Peoples R China. 地址: [ 1 ] Northeast Agr Univ, Coll Hort, Harbin, Peoples R China [ 2 ] Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou, Hainan, Peoples R China 电子邮件地址:luanfeishi@sina.com 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation of China 31071812 China Agriculture Research System CARS-26-02 查看基金资助信息 出版商 FUNPEC-EDITORA, RUA FLORIANO PEIXOTO 2444, ALTO DA BOA VISTA, RIBEIRAO PRETO, SP 00000, BRAZIL 类别 / 分类 研究方向:Biochemistry & Molecular Biology; Genetics & Heredity Web of Science 类别:Biochemistry & Molecular Biology; Genetics & Heredity 文献信息 文献类型:Article 语种:English 入藏号: WOS:000331846400006 ISSN: 1676-5680 期刊信息 Impact Factor (影响因子): Journal Citation Reports® 其他信息 IDS 号: AB5RO Web of Science 核心合集中的 "引用的参考文献": 19 Web of Science 核心合集中的 "被引频次": 0 |
2楼2014-05-23 09:39:53
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3楼2014-05-25 08:40:27