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下面是我自己翻译的，老师说太中文化了，求帮助修改下，或从新翻译， 谢谢啦！！！ 摘要：通过已知拟南芥的生长素结合蛋白（ABP1）基因序列和禾本科物种高粱、水稻、玉米、二叶短柄草的全基因组序列进行比较分析，在4个禾本科物种中鉴定其他的生长素结合蛋白基因家族并对ABP1基因家族进行序列比对分析、共线性分析、基因家族的系统进化发育分析研究该基因家族保守结构域及作用机制和该基因家族的遗传进化机制。结果显示，拟南芥、高粱、水稻、玉米、二叶短柄草ABP1蛋白中有12个同源性较高的序列基因，其多数定位于内质网上，少数位于等离子体膜上；除At4g19647295编码的蛋白跨膜外，其余皆不跨膜。12个蛋白结构保守性较强，都有Auxin_BP和Cupin_2俩个保守域位置，并分成2个亚家族。 关键词：ABP1，禾本科物种，拟南芥，结构预测，进化分析 Abstract: compare gene sequence of the Arabidopsis thaliana’s auxin binding protein (ABP1) with the whole genome sequence of the four grasses, sorghum bicolor, oryza sativa, zea mays and Brachypodium discolor .Then establish auxin binding protein gene family and use the ABP1 gene family for sequence alignment analysis, linear analysis, evolution development domain analysis to study conservative structure, mechanism of action and the genetic evolutionary mechanism of this gene family. The results showed that there are 12 members in this ABP1 gene family , and most of its location in the endoplasmic reticulum, a few on the plasma membrane; Besides the proteins encoded by At4g19647295 across the membrane, the rest are not .The conservative of these 12 protein structure is stronger, and they all possess Auxin_BP and Cupin_2 these two conservative domain; And divided into two subfamily. Keywords: ABP1, grasses, Arabidopsis thaliana, prediction of structure, evolutionary analysis |
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4楼2014-05-21 08:50:41
2楼2014-05-20 22:57:02
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爱与雨下: 金币+1 2014-05-22 07:03:16
爱与雨下: 金币+1 2014-05-22 07:03:16
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Abstract:The known gene sequences of Arabidopsis auxin binding protein (ABP1) were compared with the whole genome sequences of the four Poaceae species of sorghum vulgar , rice/oryza sativa, maize, and two leave Brachypodium distachyon .Other auxin binding protein of the four Poaceae species were identified and sequence alignment , synteny ,and gene family phylogenetic analysis of ABP1 gene family were performed to investigate the gene conserved domain and its action mechanism ,and ,genetic evolution mechanism . The results showed that Arabidopsis, sorghum, rice, maize, two leave Brachypodium ABP1 protein have 12 high homology sequence gene, the majority of which located in the endoplasmic reticulum, a few in the plasma membrane.Except At4g19647295 encoded protein, the remaining are not transmembrane protein. All 12 proteins are highly conserved structure and all have Auxin_BP and Cupin_2 two conserved domain and divided into two subfamilies. Keywords: ABP1, Poaceae species , Arabidopsis thaliana, structure prediction, evolution analysis |
3楼2014-05-21 00:05:57