| 查看: 1403 | 回复: 8 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
谁有用荧光探针测过氧化氢的文献（最好是生物体内）
|
|||
| 谁有用荧光探针测过氧化氢的文献（最好是生物体内） |
回复此楼» 猜你喜欢
一志愿中山大学生物学，295分，求A区调剂
已经有2人回复
-
求助化合物CD数据的处理
已经有0人回复
-
生物力学与组织工程学论文润色/翻译怎么收费?
已经有129人回复
-
广州医科大学生物医学工程学院 生物医学工程工学-083100 招生
已经有5人回复
-
一志愿湖南大学 生物学332分 有国奖+省级
已经有1人回复
-
美国南加大(USC)药学院Prof. Yong (Tiger) Zhang课题组诚聘博士后
已经有0人回复
-
湖北工业大学食品科学与工程系课题组招收2026级食品/生物医药硕士生（含调剂）
已经有0人回复
-
2026年博士生自我推荐
已经有1人回复
-
一志愿211生物学（0710）总分325求调剂
已经有2人回复
-
求调剂推荐
已经有2人回复
5楼2014-04-27 22:42:29
brilliant
捐助贵宾 (职业作家)
- 应助: 118 (高中生)
- 金币: 12318.9
- 散金: 262
- 红花: 47
- 帖子: 4912
- 在线: 1107.8小时
- 虫号: 71523
- 注册: 2005-05-31
- 性别: GG
- 专业: 配位化学
★ ★ ★
小木虫: 金币+0.5, 给个红包，谢谢回帖
黄酮: 金币+1, 谢谢回帖交流 2014-04-28 08:53:33
康洁: 金币+1 2014-07-23 10:23:15
小木虫: 金币+0.5, 给个红包，谢谢回帖
黄酮: 金币+1, 谢谢回帖交流 2014-04-28 08:53:33
康洁: 金币+1 2014-07-23 10:23:15
2楼2014-04-26 05:55:38
brilliant
捐助贵宾 (职业作家)
- 应助: 118 (高中生)
- 金币: 12318.9
- 散金: 262
- 红花: 47
- 帖子: 4912
- 在线: 1107.8小时
- 虫号: 71523
- 注册: 2005-05-31
- 性别: GG
- 专业: 配位化学
★ ★ ★ ★ ★ ★ ★
小木虫: 金币+0.5, 给个红包，谢谢回帖
黄酮: 金币+1, 谢谢回帖交流 2014-04-29 09:19:52
康洁(likedong1021代发): 金币+5, 感谢回帖交流！ 2014-07-28 04:51:19
小木虫: 金币+0.5, 给个红包，谢谢回帖
黄酮: 金币+1, 谢谢回帖交流 2014-04-29 09:19:52
康洁(likedong1021代发): 金币+5, 感谢回帖交流！ 2014-07-28 04:51:19
|
Nature Protocols | Protocol PrintEmailShare/bookmark Cite U LikeFacebookTwitterDeliciousDiggGoogle+LinkedInRedditStumbleUponPrevious articleNature Protocols | Protocol Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry Next articleNature Protocols | Protocol In vivo introduction of transgenes into mouse sciatic nerve cells in situ using viral vectors Synthesis, labeling and bioanalytical applications of a tris(2,2′-bipyridyl)ruthenium(II)-based electrochemiluminescence probe Xiaoming Zhou,1, Debin Zhu,1, Yuhui Liao,1, Weipeng Liu,1, Hongxing Liu,1, Zhaokui Ma1, & Da Xing1, AffiliationsContributionsCorresponding author Journal name: Nature Protocols Volume: 9, Pages: 1146–1159 Year published: (2014)DOI: doi:10.1038/nprot.2014.060 Published online17 April 2014 Article toolsFull text Synthesis, labeling and bioanalytical applications of a tris(2,2′-bipyridyl)ruthenium(II)-based electrochemiluminescence prob... Download as PDF (921 KB) View interactive PDF in ReadCube CitationReprintsRights & permissionsArticle metrics Abstract Abstract• References• Author information• Supplementary information Assays using probes labeled with electrochemiluminescent moieties are extremely powerful analytical tools that are used in fields such as medical diagnostics, environmental analysis and food safety monitoring, in which sensitive, reliable and reproducible detection of biomolecules is a requirement. The most efficient electrochemiluminescence (ECL) reaction to date is based on tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) with tripropylamine (TPrA) as the co-reactant. Here we present a detailed protocol for preparing Ru(bpy)32+ probes and their bioanalytical applications. This protocol includes (i) the synthesis of a biologically active Ru(bpy)32+-N-hydroxysuccinimide (NHS) ester, (ii) its covalent labeling with both antibodies and DNA probes and (iii) the detection and quantification of ECL in a microfluidic system with a paramagnetic microbead solid support. In our magnetic bead–based ECL system, two probes are required: a capture probe (labeled with biotin to be captured by a streptavidin-coated magnetic bead) and a detector probe (labeled with Ru(bpy)32+). The complex consisting of the analyte, the capture probe, the detector probe and the magnetic bead is brought into contact with the electrode by using a magnetic field. The Ru(bpy)32+ reacts with TPrA in solution to generate the ECL signal. The full protocol, including the synthesis and labeling of the bioactive Ru(bpy)32+, requires 5–6 d to complete. ECL immunoassays or nucleic acid tests only require 1.5–2 h, including the sample preparation time. |
6楼2014-04-28 09:28:22
zqx1990
木虫 (正式写手)
- 应助: 49 (小学生)
- 金币: 4871.7
- 散金: 350
- 红花: 7
- 帖子: 723
- 在线: 600.9小时
- 虫号: 1264137
- 注册: 2011-04-13
- 性别: GG
- 专业: 生物医用高分子材料
★ ★
小木虫: 金币+0.5, 给个红包，谢谢回帖
黄酮: 金币+1, 谢谢回帖交流 2014-04-29 09:20:57
小木虫: 金币+0.5, 给个红包，谢谢回帖
黄酮: 金币+1, 谢谢回帖交流 2014-04-29 09:20:57
7楼2014-04-28 22:00:48
