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清清苹果香

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[求助] 论文摘要翻译

本文筛选了纯化天然植物水蓼(Polygonum hydropiper L)中总黄酮的最佳大孔树脂及其最优工艺条件,采用单因素试验考察了树脂种类、上样液浓度、上样液pH、上样流速、洗脱液浓度、洗脱流速、树脂动态吸附、解析和树脂的重复使用次数等因素对纯化的影响。用紫外分光光度法测定总黄酮的含量,计算吸附量解、解析率和提取液总黄酮的含量,最后通过验证试验确定最佳工艺条件为树脂种类为ADS-7,上样液质量浓度0.8856 mg/mL,pH值为4.48、,树脂的上样量5 mL/g;以50%的乙醇洗脱,流速3 mL/min,洗脱液用量300 mL。经ADS-7型树脂处理后的总黄酮质量分数达到76.43%,收率为93.04%。结论:ADS-7型大孔树脂分离纯化水蓼黄酮效果最佳,是一种理想的分离纯化介质。


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leilejiayou

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RXMCDM: 金币+1 2014-04-26 20:09:14
清清苹果香(RXMCDM代发): 金币+12 2014-06-04 09:27:11
This paper screened  the best macroporous resin and its optimum conditions in the process of purifying total flavonoids in natural plant water smartweed (Polygonum hydropiper L) , used single-factor tests to examine the effects of type of resin, sample concentration, pH of the sample , the sample flow rate, eluent concentration, elution rate, dynamic adsorption resin, number of repeated use and other factors on purification.Using UV spectrophotometry to examine the content of total flavonoids, then calculated the amount of adsorption solution, parsing and extraction rate of total flavonoids content, finally through the validated tests to determine the optimum conditions are as followed:the type of resin is ADS-7, the sample solution concentration is 0.8856 mg / mL, the value of pH is 4.48, the resin sample volume is 5 mL / g; eluted with 50% ethanol, at a flow rate of 3 mL / min,  eluent consumes 300 mL. After the treatment of ADS-7 resin,mass fraction of total flavonoids reaches 76.43%, the yield is 93.04%.
Conclusion: ADS-7 macroporous resin does the best in the process of separation and purification of water smartweed flavonoids, it is an ideal medium in separation and purification.

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4楼2014-04-26 20:08:22
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conf20140319

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RXMCDM: 屏蔽内容 2014-04-25 15:35:48
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3楼2014-04-25 15:03:35
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