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2.5. Color, browning index and hidroxymethylfurfural determination Color was determined using a Hunter Labscan II spectrophotometric colorimeter (Hunter associates laboratory Inc., Reston, Virginia, USA) controlled by a computer that calculated color ordinates from the reflectance spectrum (Calvo & Duran, 1998) and calibrated with a white standard tile. Samples were placed in Petri dishes and filled to the top, and color was recorded using the CIELab uniform color space at room temperature. Color determined by CIE (Commission Internationale l¡¯Eclairage) classifies color in three dimensions; L , brightness, a , red to green color and b , yellow to blue color. Chroma ((a + b ) ), that quantify color intensity, and total color differences ( E = L + a + b ), which indicate the magnitude of the color difference between juices at initial time and after the storage period, were also determined. The results were expressed in accordance with the CIELAB system with reference to illuminant D65 and with a visual angle of 10¡æ. The measurements were made in triplicate. Browning index: Non-enzymatic browning index is determined with an absorbance measure at 420 nm. Three milliliter of orange juice are centrifuged at 2000 rpm during 20 min at 18¡æ, and the supernatant is mixed equally distributed with ethanol 95%. After filtering the mixture, the absorbance is measured at 420 nm (Meydav, Saguy, & Kopelman, 1977). Hidroxymethylfurfural (HMF): Hidroxymethylfurfural content is measured using the method described by IFFJP (1984). This method is based in that HMF reacts with barbituric acid and p-toluidin, forming a red compound. This reaction has a maximum at 3¨C4 min. The Association ofthe Industry of Juices and Nectars from Fruits and Vegetables of the European Economic Community (AIJN, 1996) has included the amount of HMF among the absolute parameters of quality (620 mg/L) in the code of practice for the evaluation of fruits and vegetable juices. |
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