图片下面的标注: Several PPXY motif-containing proteins
were identified by large-scale coimmunoprecipitation. A total of 293 cells expressing 3XFlag-6XHis-TAZ, -S89A and -WWm (together
with cells transfected with vector) were lysed and the lysates were processed for large-scale coimmunoprecipitation. The precipitates
were resolved by SDS–PAGE and the gel pieces were excised out for mass spectrometry (MS) analysis. The bands containing Amot,
AmotL1, TAZ, Wbp2 and their MS coverage of the polypeptide were indicated (left). The Wbp2 peptide sequencescovered are shown
(right).
FJ0EBWZF0XTB5MM4BOMKNTV.jpg