24小时热门版块排行榜    

Znn3bq.jpeg
查看: 3208  |  回复: 9
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

1970-01-01 08:00:00
已阅   申请MicEPI   回复此楼   编辑   查看我的主页

智能机器人

Robot (super robot)

我们都爱小木虫

ych518

木虫 (小有名气)

【答案】应助回帖


感谢参与,应助指数 +1
sjyllh(dllchina代发): 金币+1, 鼓励应助 2014-03-08 00:22:22
PAO1分布极为广泛,而且是条件致病菌,研究很多。就像模式菌一样,很容易买到

[ 发自小木虫客户端 ]
假单胞菌
7楼2014-03-07 22:22:53
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 10 个回答

zhaozhibozhi

金虫 (正式写手)

【答案】应助回帖

★ ★
感谢参与,应助指数 +1
dllchina: 金币+2, 多多交流 2014-03-25 08:51:15
几种突变体?这个没法说,太泛了。

PAO1是铜绿假单胞菌的一个菌株,已测全基因组。
做一个阳光、开朗的小和尚
2楼2014-03-06 11:18:45
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

sjyllh

铜虫 (初入文坛)

引用回帖:
2楼: Originally posted by zhaozhibozhi at 2014-03-06 11:18:45
几种突变体?这个没法说,太泛了。

PAO1是铜绿假单胞菌的一个菌株,已测全基因组。

我看到文献里用到的都是PAO1菌株,想问你一下,PAO1有什么特点吗?为什么都用它做实验。谢谢!
天道酬勤,乐战不殆!
3楼2014-03-06 12:13:44
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zhaozhibozhi

金虫 (正式写手)

★ ★ ★
dllchina: 金币+3, good 2014-03-06 12:58:24
引用回帖:
3楼: Originally posted by sjyllh at 2014-03-06 12:13:44
我看到文献里用到的都是PAO1菌株,想问你一下,PAO1有什么特点吗?为什么都用它做实验。谢谢!...

Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of the original Australian PAO isolate, has been distributed worldwide to laboratories and strain collections.

The common reference strain is P. aeruginosa PAO1, a spontaneous chloramphenicol-resistant mutant of the original PAO strain (earlier called “P. aeruginosa strain 1”) that had been isolated in 1954 from a wound in Melbourne, Australia (9, 10). This PAO1 strain from Bruce Holloway's laboratory has become the reference strain for Pseudomonas genetics and functional analyses of the physiology and metabolism of this gammaproteobacterium. A genetic map of its chromosome was generated by exploiting the mechanisms of gene exchange in bacteria, i.e., transduction and conjugation (11). With the advent of pulsed-field gel electrophoresis (PFGE), a physical map of the PAO1 genome was constructed (32) and later merged with the genetic map information (12). By 2000 the PAO1 strain had been completely sequenced (36). Thereafter, the genome annotation has been continually updated and the database content and functionality have been expanded to facilitate accelerated discovery of P. aeruginosa drug targets and vaccine candidates (38). Two near-saturation libraries of transposon insertion mutants have been constructed in P. aeruginosa PAO1 as a global resource for the scientific community (14, 22).
参照上述。

» 本帖已获得的红花(最新10朵)

做一个阳光、开朗的小和尚
4楼2014-03-06 12:34:08
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 一志愿安大生物学07初试322、本科二本、调剂求助 +10 李多米lee. 2026-04-12 11/550 2026-04-12 22:58 by yuyin1233
[考研] 食品与营养(0955)271求调剂 +7 升格阿达 2026-04-12 7/350 2026-04-12 22:56 by yuyin1233
[考研] 一志愿中南大学 0855 机械 286 求调剂 +10 不会吃肉 2026-04-12 10/500 2026-04-12 22:51 by 零零二
[考研] 求调剂,985材料与化工348分 +9 涵竹刘 2026-04-11 13/650 2026-04-12 22:40 by 涵竹刘
[考研] 药学求调剂 +3 RussHu 2026-04-12 4/200 2026-04-12 17:49 by 陈皮皮
[考研] 一志愿双非085400电子信息344 求调剂,对材料和化学方向也感兴趣 +11 无情的小羊 2026-04-09 12/600 2026-04-12 17:41 by 686666
[考研] 286求调剂 +26 Faune 2026-04-06 26/1300 2026-04-12 10:24 by lhj2009
[考研] 267求调剂 +8 再忙也要吃饭啊 2026-04-09 8/400 2026-04-11 21:42 by cfdbai
[考研] 293求调剂 +8 勇远库爱314 2026-04-06 8/400 2026-04-11 20:25 by 蓝云思雨
[考研] 270求调剂 +14 杨乐369 2026-04-11 14/700 2026-04-11 20:16 by 蓝云思雨
[考研] 292求调剂 +9 笑笑袁 2026-04-09 9/450 2026-04-10 10:05 by LHGeng
[考研] 初试分332,一志愿报考西北工业大学, +11 故人?? 2026-04-09 11/550 2026-04-09 21:54 by JineShine
[考研] 求机械专硕297第二批调剂 +5 拾柒12。 2026-04-08 5/250 2026-04-09 16:43 by 允当适度
[考研] 085404,334分,求调剂 +5 sunjie8888 2026-04-08 8/400 2026-04-09 07:26 by sunjie8888
[考研] 二次调剂求老师收留 +3 笑笑袁 2026-04-08 3/150 2026-04-08 23:50 by 醉在风里
[考研] 263分B区求调剂 +6 李nihao 2026-04-08 6/300 2026-04-08 09:38 by 南开小綦
[考研] 生物医药调剂|SCI中科院三区一作+多项科研成果 +8 likangxing 2026-04-07 11/550 2026-04-08 00:02 by lys0704
[考研] 材料工程专业日语生求调剂 +9 111623 2026-04-07 9/450 2026-04-07 23:31 by 一只好果子?
[考研] 316求调剂 +4 15318418673 2026-04-07 4/200 2026-04-07 22:12 by hemengdong
[考研] 生物学363调剂求助 +7 fanzhang6666 2026-04-06 9/450 2026-04-07 17:37 by lijunpoly
信息提示
请填处理意见