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小木虫论坛-学术科研互动平台 » 生物医药区 » 微生物 » 综合其他 » 铜绿假单胞菌有几种突变体啊？另外铜绿假单胞菌PAO1是什么啊？
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sjyllh

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4楼: Originally posted by zhaozhibozhi at 2014-03-06 12:34:08
Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of the original Australian  ...

谢谢！还想问你下从哪里得到这种菌株啊？
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5楼2014-03-06 20:32:10
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zhaozhibozhi

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dllchina: 金币+2, 多多交流 2014-03-25 08:51:15
几种突变体？这个没法说，太泛了。

PAO1是铜绿假单胞菌的一个菌株，已测全基因组。
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2楼2014-03-06 11:18:45
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sjyllh

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2楼: Originally posted by zhaozhibozhi at 2014-03-06 11:18:45
几种突变体？这个没法说，太泛了。

PAO1是铜绿假单胞菌的一个菌株，已测全基因组。

我看到文献里用到的都是PAO1菌株，想问你一下，PAO1有什么特点吗？为什么都用它做实验。谢谢！
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3楼2014-03-06 12:13:44
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zhaozhibozhi

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dllchina: 金币+3, good 2014-03-06 12:58:24
引用回帖:
3楼: Originally posted by sjyllh at 2014-03-06 12:13:44
我看到文献里用到的都是PAO1菌株，想问你一下，PAO1有什么特点吗？为什么都用它做实验。谢谢！...

Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of the original Australian PAO isolate, has been distributed worldwide to laboratories and strain collections.

The common reference strain is P. aeruginosa PAO1, a spontaneous chloramphenicol-resistant mutant of the original PAO strain (earlier called “P. aeruginosa strain 1”) that had been isolated in 1954 from a wound in Melbourne, Australia (9, 10). This PAO1 strain from Bruce Holloway's laboratory has become the reference strain for Pseudomonas genetics and functional analyses of the physiology and metabolism of this gammaproteobacterium. A genetic map of its chromosome was generated by exploiting the mechanisms of gene exchange in bacteria, i.e., transduction and conjugation (11). With the advent of pulsed-field gel electrophoresis (PFGE), a physical map of the PAO1 genome was constructed (32) and later merged with the genetic map information (12). By 2000 the PAO1 strain had been completely sequenced (36). Thereafter, the genome annotation has been continually updated and the database content and functionality have been expanded to facilitate accelerated discovery of P. aeruginosa drug targets and vaccine candidates (38). Two near-saturation libraries of transposon insertion mutants have been constructed in P. aeruginosa PAO1 as a global resource for the scientific community (14, 22).
参照上述。
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