24小时热门版块排行榜    

查看: 3496  |  回复: 9
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

1970-01-01 08:00:00
已阅   申请MicEPI   回复此楼   编辑   查看我的主页

智能机器人

Robot (super robot)

我们都爱小木虫

颜瑜逐月

铁杆木虫 (著名写手)

lz,请问您买到铜绿假单胞菌PAO1了吗?求了很多单位都没买到啊,您还有没有保存的?不胜感激啊!
元知造物心腸別,老卻英雄似等閒。
10楼2014-08-02 11:47:31
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 10 个回答

zhaozhibozhi

金虫 (正式写手)

【答案】应助回帖

★ ★
感谢参与,应助指数 +1
dllchina: 金币+2, 多多交流 2014-03-25 08:51:15
几种突变体?这个没法说,太泛了。

PAO1是铜绿假单胞菌的一个菌株,已测全基因组。
做一个阳光、开朗的小和尚
2楼2014-03-06 11:18:45
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

sjyllh

铜虫 (初入文坛)

引用回帖:
2楼: Originally posted by zhaozhibozhi at 2014-03-06 11:18:45
几种突变体?这个没法说,太泛了。

PAO1是铜绿假单胞菌的一个菌株,已测全基因组。

我看到文献里用到的都是PAO1菌株,想问你一下,PAO1有什么特点吗?为什么都用它做实验。谢谢!
天道酬勤,乐战不殆!
3楼2014-03-06 12:13:44
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zhaozhibozhi

金虫 (正式写手)

★ ★ ★
dllchina: 金币+3, good 2014-03-06 12:58:24
引用回帖:
3楼: Originally posted by sjyllh at 2014-03-06 12:13:44
我看到文献里用到的都是PAO1菌株,想问你一下,PAO1有什么特点吗?为什么都用它做实验。谢谢!...

Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of the original Australian PAO isolate, has been distributed worldwide to laboratories and strain collections.

The common reference strain is P. aeruginosa PAO1, a spontaneous chloramphenicol-resistant mutant of the original PAO strain (earlier called “P. aeruginosa strain 1”) that had been isolated in 1954 from a wound in Melbourne, Australia (9, 10). This PAO1 strain from Bruce Holloway's laboratory has become the reference strain for Pseudomonas genetics and functional analyses of the physiology and metabolism of this gammaproteobacterium. A genetic map of its chromosome was generated by exploiting the mechanisms of gene exchange in bacteria, i.e., transduction and conjugation (11). With the advent of pulsed-field gel electrophoresis (PFGE), a physical map of the PAO1 genome was constructed (32) and later merged with the genetic map information (12). By 2000 the PAO1 strain had been completely sequenced (36). Thereafter, the genome annotation has been continually updated and the database content and functionality have been expanded to facilitate accelerated discovery of P. aeruginosa drug targets and vaccine candidates (38). Two near-saturation libraries of transposon insertion mutants have been constructed in P. aeruginosa PAO1 as a global resource for the scientific community (14, 22).
参照上述。

» 本帖已获得的红花(最新10朵)

做一个阳光、开朗的小和尚
4楼2014-03-06 12:34:08
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[找工作] 售SCI文章,我:8O5.5.1.O.54,科目齐全,可+急 +4 k0dTPqJtl0jt 2026-08-14 4/200 2026-08-15 02:21 by 4wMiSEwB6436
[教师之家] 售SCI一区T0P文章,我:8O.55.1.O.5.4,科目齐全,可+急 +4 7lpolszZVXgi 2026-08-14 4/200 2026-08-15 01:08 by 4wMiSEwB6436
[基金申请] 是这周出结果还是下周出结果? +4 yuleib84 2026-08-11 4/200 2026-08-14 23:05 by lfy8008
[教师之家] 售SCI一区文章,我:8.O.55.1.O.54,科目齐全,可伽急 +3 HFw0lei2R37i 2026-08-14 5/250 2026-08-14 22:32 by 4wMiSEwB6436
[基金申请] filecode +6 cratir 2026-08-14 10/500 2026-08-14 18:29 by 笑叹辞穷
[基金申请] 奇怪,两个人的filecode固定段从头到尾一模一样 +8 布布和一二 2026-08-10 11/550 2026-08-14 14:58 by Equinoxhua
[基金申请] 有时候,自然基金真的不能太认真 (我的申报经验) +6 majunge000 2026-08-11 8/400 2026-08-14 14:44 by 医学老男孩
[基金申请] 好奇怪的filecode +6 布布和一二 2026-08-08 7/350 2026-08-14 13:38 by Equinoxhua
[硕博家园] 请教兼职经验 +3 是阿文鸭 2026-08-09 3/150 2026-08-14 12:07 by HER12025
[硕博家园] 读博的好处 +4 lnee 2026-08-11 4/200 2026-08-14 10:20 by ahsoarli
[基金申请] 我的国基提前知道中了,可是同事的操作让我实在接受不了,怎么会有这样的人 +10 家与远方 2026-08-10 15/750 2026-08-14 02:08 by 绵羊哥哥
[文学芳草园] 阿姨 +4 汪汪锅 2026-08-09 4/200 2026-08-13 19:43 by arzu_hma
[基金申请] 重要来源:本周末出结果 +10 瞬息宇宙 2026-08-12 10/500 2026-08-13 15:46 by likettle
[基金申请] 不应该看fileCode +7 且听虎啸 2026-08-12 9/450 2026-08-13 14:27 by flydreamws
[基金申请] Filecode 又变了,巨变 +3 WH3796 2026-08-12 4/200 2026-08-13 14:13 by 小木虫6752397
[基金申请] 综述论文作为代表作会不会影响评审专家的印象分? +11 yufeiwaner 2026-08-09 13/650 2026-08-12 08:17 by yufeiwaner
[基金申请] 为什么网上很多人说本周 12号出结果 +6 瞬息宇宙 2026-08-10 7/350 2026-08-11 19:25 by Tide man
[基金申请] 据悉今年马上要出结果了 +7 瞬息宇宙 2026-08-10 8/400 2026-08-10 12:42 by Vivilian
[基金申请] 这样的filecode谁见过 +11 布布和一二 2026-08-08 22/1100 2026-08-10 11:10 by wmfsnow
[基金申请] fileCode有新解读? +10 Tide man 2026-08-08 18/900 2026-08-09 12:55 by 仁砚薪传
信息提示
请填处理意见