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2.3. Thermal treatment To treat samples ARMFIELD FT74P equipment with a plate exchanger was used. Juice placed in a feeding tank was impulsed by a pump to the heat exchanger where the treatment conditions (90℃, 20 s) are reached. Heating of orange juice at 90–99℃ for 15–30 s is normal in commercial practice (Braddock, 1999). After treatment, the juice was cooled with cold water from a cooler (ARMFIELD FT61), and it was packed aseptically and stored at 2℃ and 10℃ until analysis. 2.4. Storage conditions Juice samples were packaged in Elopack packages (purepack ), and they were stored in refrigeration and darkness at 2℃and 10℃ (±1℃) with controlled humidity. Samples were analyzed in duplicate immediately after processing, then after 1, 2, 3, 4, 6, and 7 weeks of storage. 2.6. Physicochemical determinations pH determination was based on the potentiometric measurement at 20℃. It was determined in a Crison GLP 21 pH meter equipped with a temperature compensation sensor at 20℃. Brix was determined by measurement of the refraction index with an Atago model RX-1000 digital refractometer at 20℃. Physicochemical determinations of the samples were analyzed in triplicate. 2.7. Statistical analysis Results were compared using one-way analysis of variance (ANOVA). Tukey test (p < 0.05) was applied to compare the average values obtained. The computer program employed was SPSS (Statistical Package for the Social Sciences) 12.0 for Windows. |
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2.3。热处理 处理样品使用带有平板式热交换器的ARMFIELD FT74P设备。放在进料罐中的果汁通过泵冲向达到（90℃，20秒）的热交换器。按通常的商业惯例（Braddock,1999），橙汁在90-99℃加热15-30 s。处理后的果汁用来自冷却器（ARMFIELD FT61）冷水冷却，然后在无菌条件下包装，贮存于2℃和10℃，直到分析。 2.4。储存条件 果汁样品包装于Elopack包（purepack？），存放冰箱，黑暗条件2℃和10℃（±1℃）并控制湿度。处理后，立即双份样品进行分析，再贮藏1，2，3，4，6，和7周。 2.6。物理化学性质 在20℃用电位测量进行 pH值测定。方法为用带温度补偿传感器的CRISON GLP21 pH计在20℃测定。 糖度由Atago RX-1000型 数字折射仪在20℃测定折射率测出。样品的物理化学性质以一式三份进行分析测定。 2.7。统计分析 结果用单因素方差分析（ANOVA）进行比较。 应用Tukey检验（P <0.05）比较所获得的平均值。使用的计算机程序是Windows SPSS （Statistical Package for the Social Sciences，社会科学统计软件包）12.0。 |
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