| ²é¿´: 673 | »Ø¸´: 4 | ||
| µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû | ||
zhhuijunľ³æ (ÕýʽдÊÖ)
|
[ÇóÖú]
°ïæ²éSCIÊÕ¼ÁËûÓÐ
|
|
|
ÄÄλÅóÓѰïÎÒ²éÒ»ÏÂÎÄÕÂAn efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.) H.J. Zhang, P. Gao1, X.Z. Wang1 and F.S. Luan SCIÊÕ¼ÁËûÓС£Ð»Ð»°¡¡£ |
» ²ÂÄãϲ»¶
081700 µ÷¼Á 267·Ö
ÒѾÓÐ8È˻ظ´
Çë½ÌÏ´ó¼Ò 2026Äê¹ú¼Ò»ù½ðÉêÇëÊÇ˫äÉóÂð£¿
ÒѾÓÐ5È˻ظ´
276Çóµ÷¼Á¡£ÓаëÄêµç³ØºÍ°ëÄê¸ß·Ö×Óʵϰ¾Àú
ÒѾÓÐ10È˻ظ´
Ò»Ö¾Ô¸ÄϺ½²ÄÁÏר317·ÖÇóµ÷¼Á
ÒѾÓÐ4È˻ظ´
Çóµ÷¼Á
ÒѾÓÐ4È˻ظ´
ÉúÎïѧѧ˶Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
284Çóµ÷¼Á
ÒѾÓÐ10È˻ظ´
һ־Ըɽ¶«´óѧҩѧѧ˶Çóµ÷¼Á
ÒѾÓÐ4È˻ظ´
07»¯Ñ§280·ÖÇóµ÷¼Á
ÒѾÓÐ4È˻ظ´
298-Ò»Ö¾Ô¸Öйúũҵ´óѧ-Çóµ÷¼Á
ÒѾÓÐ12È˻ظ´
zhhuijun
ľ³æ (ÕýʽдÊÖ)
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 4817.5
- É¢½ð: 19
- ºì»¨: 3
- Ìû×Ó: 434
- ÔÚÏß: 293.9Сʱ
- ³æºÅ: 865499
- ×¢²á: 2009-10-08
- ÐÔ±ð: GG
- רҵ: Êß²ËѧÓë¹Ï¹ûѧ
3Â¥2014-02-16 11:01:15
jssxh
ÖÁ×ðľ³æ (ÖªÃû×÷¼Ò)
- LS-EPI: 30
- Ó¦Öú: 64 (³õÖÐÉú)
- ¹ó±ö: 0.273
- ½ð±Ò: 44678.6
- É¢½ð: 605
- ºì»¨: 98
- Ìû×Ó: 5844
- ÔÚÏß: 710.4Сʱ
- ³æºÅ: 233249
- ×¢²á: 2006-03-29
- רҵ: ÓлúºÏ³É
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
gruyclewee: ½ð±Ò+3, ¸ÐлӦÖú£¬½±ÀøÒ»Ï¡£ 2014-02-15 10:10:14
zhhuijun: ½ð±Ò+5, ¡ï¡ï¡ïºÜÓаïÖú, лл°¡ 2014-02-16 10:59:46
gruyclewee: ½ð±Ò+3, ¸ÐлӦÖú£¬½±ÀøÒ»Ï¡£ 2014-02-15 10:10:14
zhhuijun: ½ð±Ò+5, ¡ï¡ï¡ïºÜÓаïÖú, лл°¡ 2014-02-16 10:59:46
|
An efficient selection and regeneration protocol for Agrobacterium-mediated transformation of oriental melon (Cucumis melo L. var. makuwa) ×÷Õß:Choi, JY (Choi, Jun Young)[ 1,2 ] ; Shin, JS (Shin, Jeong Sheop)[ 2 ] ; Chung, YS (Chung, Young Soo)[ 3 ] ; Hyung, NI (Hyung, Nam-In)[ 1 ] PLANT CELL TISSUE AND ORGAN CULTURE ¾í: 110 ÆÚ: 1 Ò³: 133-140 DOI: 10.1007/s11240-012-0137-6 ³ö°æÄê: JUL 2012 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation using cotyledon explants of oriental melon (Cucumis melo L. var. makuwa) has been developed. All six oriental melon cultivars evaluated in the study showed a > 90 % shoot regeneration frequency and produced 1.8-3.6 shoots per cotyledon explant when cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg L-1 benzyladenine and 0.01 mg L-1 indoleacetic acid. Kanamycin (Km) and geneticin (Gt) in the shoot induction medium (SIM) were compared both qualitatively and quantitatively for their efficiency as a selection agent for the selection and regeneration of transgenic plants after Agrobacterium-mediated transformation. Shoot formation was completely inhibited at 50 mg L-1 Km and 10 mg L-1 Gt. Relatively high concentrations of both Gt and Km (> 100 mg L-1 Km and > 25 mg L-1 Gt) were necessary because large numbers of non-transgenic shoots survived during the selection process. The incorporation of a selectable marker (neomycin phosphotransferase II) into the genome of transgenic plants was confirmed using beta-glucuronidase (GUS), PCR and Southern blot analysis. Shoot regeneration frequencies were 41.2 % at 100 mg L-1 Km and 15.2 % at 30 mg L-1 Gt 8 weeks after transformation, whereas the transformation frequencies based on the PCR were 2.9 and 7.1 %, respectively, 16 weeks after transformation. These results demonstrate that a large portion of the regenerated shoots on SIM supplemented with 100 mg L-1 Km consisted of non-transformed or escaped shoots, indicating that 30 mg L-1 Gt is the more suitable for the selection and regeneration of transgenic plants in oriental melon. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Oriental melon; Transformation; Kanamycin; Geneticin; Selection KeyWords Plus:COLOCYNTHIS-CITRULLUS L.; PLANT-REGENERATION; SHOOT REGENERATION; GENETIC-TRANSFORMATION; LEAF EXPLANTS; ORGANOGENESIS; RESISTANCE; CULTIVARS; MUSKMELON; GENOTYPE ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Hyung, NI (ͨѶ×÷Õß) Sangmyung Univ, Dept Plant Sci & Technol, Cheonan 330720, South Korea. µØÖ·: [ 1 ] Sangmyung Univ, Dept Plant Sci & Technol, Cheonan 330720, South Korea [ 2 ] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea [ 3 ] Dong A Univ, Dept Genet Engn, Pusan 604714, South Korea µç×ÓÓʼþµØÖ·:nihyung@smu.ac.kr ³ö°æÉÌ SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS Àà±ð / ·ÖÀà Ñо¿·½Ïò:Biotechnology & Applied Microbiology; Plant Sciences Web of Science Àà±ð:Biotechnology & Applied Microbiology; Plant Sciences ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000304664300014 ISSN: 0167-6857 ÆäËûÐÅÏ¢ IDS ºÅ: 950QR Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 35 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 3 |
2Â¥2014-02-15 08:51:41
hezhao311
ÖÁ×ðľ³æ (ÖøÃûдÊÖ)
- LS-EPI: 12
- Ó¦Öú: 9 (Ó×¶ùÔ°)
- ¹ó±ö: 0.027
- ½ð±Ò: 24369.4
- ºì»¨: 14
- ɳ·¢: 2
- Ìû×Ó: 2513
- ÔÚÏß: 313.3Сʱ
- ³æºÅ: 819621
- ×¢²á: 2009-07-31
- ÐÔ±ð: GG
- רҵ: ×÷ÎïÖÖÖÊ×ÊÔ´ÓëÒÅ´«ÓýÖÖѧ
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
| ²é²»µ½ÄãÒªµÄÎÄÕ¡£Ö»ÓÐ2#Â¥ÌṩµÄÄÇÆª¡£¼ûÁ´½Óhttp://apps.webofknowledge.com/S ... 0-ba51-4b760fef15ea |
4Â¥2014-02-17 11:41:17
zhhuijun
ľ³æ (ÕýʽдÊÖ)
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 4817.5
- É¢½ð: 19
- ºì»¨: 3
- Ìû×Ó: 434
- ÔÚÏß: 293.9Сʱ
- ³æºÅ: 865499
- ×¢²á: 2009-10-08
- ÐÔ±ð: GG
- רҵ: Êß²ËѧÓë¹Ï¹ûѧ
5Â¥2014-02-17 16:21:19













»Ø¸´´ËÂ¥
10