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[ÇóÖú] PLOS one ÌáÎÊ£¬ÊDZ»¾ÜµÄǰ×àÂ𣿠ÒÑÓÐ6È˲ÎÓë

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> At this stage, we would request you report additional details relating to the in vivo experiments described in your submission, particularly the survival study:
> 1. Please specify whether the rats died as a direct result of the experimental procedures, or whether humane endpoints were used during the survival study (i.e., the animals were humanely euthanized when they met certain clinical criteria)
> a. If humane endpoints were used, please specify the criteria used to determine when the animals should be humanely euthanized
> b. Please specify how frequently the condition of the animals was monitored
> 2. Please specify the method of sacrifice used for each experiment
> 3. Please provide details of any steps taken to minimize suffering of the rats, including analgesics and anaesthetics, for each experiment
> 4. Please follow the ARRIVE guidelines for reporting animal studies (http://www.nc3rs.org.uk/page.asp?id=1357)
>
>
> Your manuscript has been returned to your account. Please log on to PLOS Editorial Manager at http://pone.edmgr.com/ to access your manuscript.
>
> Your manuscript can be found in the "Submissions Sent Back to Author" link under the New Submissions menu. After you have made the changes requested above, please be sure to view and approve the revised PDF after rebuilding the PDF to complete the resubmission process.
>
> Please note that these changes have been requested to comply with submission guidelines and your manuscript will *not* be sent to review until you have fully adhered to our requests. Once your paper has been seen by an Editor we may return it to you for further information or amendments.
>
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