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DLL4 expression in cancer cell membranes was evaluated,and averaged in ten different high power magnified fields(¡Á 400). DLL4 expression was identified in the cytoplasm and cellular membrane of cancer cells , and in the stromal cells . Ten representative tissue sections were observed by light microscropy and the percentage of DLL4 positive cancer cells was scored,averaged, and scored semiquantitatively. All immunostained slides were evaluated by two independent observers (SI and AT), who were unaware of the clinical data and disease outcome. If more than 10% of dominant staining intensity in tumor cells or stromal cells was identified, the patients were regarded as DLL4 positive. After evaluation,patients were divided into two groups according to DLL4 expression positivity. Clinicopathological factors of gastric cancer were assessed according to the General Rules of Gastric Cancer in Japan . |
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