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Spectroscopic Studies on the Interaction of Hemin with Human Telomeric G-Quadruplex DNA
XU-JIAN LUO;  QI-PIN QIN;  KE-BIN HUANG; YAN-CHENG LIU; HONG LIANG
Asian J. Chem., 2013, 25(11), pp 6396-6400
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Spectroscopic Studies on the Interaction of Hemin with Human Telomeric G-Quadruplex DNA



×÷Õß: Luo, XJ (Luo, Xu-Jian)[ 1,2 ] ; Qin, QP (Qin, Qi-Pin)[ 2 ] ; Huang, KB (Huang, Ke-Bin)[ 2 ] ; Liu, YC (Liu, Yan-Cheng)[ 2 ] ; Liang, H (Liang, Hong)[ 1,2 ]



À´Ô´³ö°æÎï: ASIAN JOURNAL OF CHEMISTRY  ¾í:25   ÆÚ:11   Ò³:6396-6400   ×Ó¼­:A   ³ö°æÄê:SEP 2013



±»ÒýƵ´Î: 0 (À´×Ô Web of Science)



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ÕªÒª: The interaction behaviours of hemin as a ligand binding with three types of human telomeric G-quadruplex DNA were examined by UV-visible and circular dichroism spectroscopic methods. The circular dichroism spectra of these three types of G-quadruplex DNA all showed major positive absorption peaks at ca. 290 nm with shoulder peaks at ca. 270 nm, along with a negative absorption peak at ca. 250 nm. The presence of hemin did not induce obvious circular dichroism spectral changes of these G-quadruplex DNA, which indicates that hemin is able to stabilize human telomeric antiparallel G-quadruplex DNA structure. For comparison, the addition of hemin induced obvious increment on the characteristic antiparallel circular dichroism absorption of the corresponding single-chain human telomeric sequences, which suggests that hemin may induce and stabilize the formation of antiparallel G-quadruplex DNA even in the absence of K+. On the other hand, the addition of the three types of G-quadruplex DNA into the hemin solution with gradually increasing concentrations induced significant hypochromicity (31.86-47.26 %) along with 11-23 nm of bathochromicity on the UV-visible absorption of hemin. From this result, it is suggested that the high binding affinity of hemin to antiparallel G-quadruplex DNA is based on the intensive intercalative binding mode of hemin to antiparallel G-quadruplex DNA by the pi-pi stacking between the macrocyclic aromatic hemin and the guanine quartet.



Èë²ØºÅ:WOS:000325089600119



ÎÄÏ×ÀàÐÍ: Article



ÓïÖÖ: English



×÷Õ߹ؼü´Ê: G-quadruplex DNA; Hemin; Telomeric DNA; Intercalative binding



KeyWords Plus: INHIBITOR; COMPLEXES; SEQUENCE; BINDING; LIGAND



ͨѶ×÷ÕßµØÖ·: Liu, YC (ͨѶ×÷Õß)




Guangxi Normal Univ, State Key Lab Cultivat Base Chem & Mol Engn Med R, Sch Chem & Chem Engn, Guilin 541004, Peoples R China.






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