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ÇëÄÄÎ»ÍøÓѰïæ²éÒ»ÏÂÏÂÁÐ1ƪ»áÒéÂÛÎÄÓÐûÓб»SCIÊÕ¼£¬Ð»Ð»£¡ Spectroscopic Studies on the Interaction of Hemin with Human Telomeric G-Quadruplex DNA XU-JIAN LUO; QI-PIN QIN; KE-BIN HUANG; YAN-CHENG LIU; HONG LIANG Asian J. Chem., 2013, 25(11), pp 6396-6400 |
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baiyuefei
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- LS-EPI: 1647
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2Â¥2013-12-20 16:34:53
baiyuefei
°æÖ÷ (ÎÄѧ̩¶·)
·çÑ©
- LS-EPI: 1647
- Ó¦Öú: 4642 (¸±½ÌÊÚ)
- ¹ó±ö: 46.97
- ½ð±Ò: 658598
- É¢½ð: 11616
- ºì»¨: 995
- ɳ·¢: 81
- Ìû×Ó: 69427
- ÔÚÏß: 13331.5Сʱ
- ³æºÅ: 676696
- ×¢²á: 2008-12-18
- ÐÔ±ð: GG
- רҵ: ºÏ³ÉÒ©Îﻯѧ
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Spectroscopic Studies on the Interaction of Hemin with Human Telomeric G-Quadruplex DNA ×÷Õß: Luo, XJ (Luo, Xu-Jian)[ 1,2 ] ; Qin, QP (Qin, Qi-Pin)[ 2 ] ; Huang, KB (Huang, Ke-Bin)[ 2 ] ; Liu, YC (Liu, Yan-Cheng)[ 2 ] ; Liang, H (Liang, Hong)[ 1,2 ] À´Ô´³ö°æÎï: ASIAN JOURNAL OF CHEMISTRY ¾í:25 ÆÚ:11 Ò³:6396-6400 ×Ó¼:A ³ö°æÄê:SEP 2013 ±»ÒýƵ´Î: 0 (À´×Ô Web of Science) ÒýÓõIJο¼ÎÄÏ×: 20 [ ²é¿´ Related Records ] ÒýÖ¤¹ØÏµÍ¼ ÕªÒª: The interaction behaviours of hemin as a ligand binding with three types of human telomeric G-quadruplex DNA were examined by UV-visible and circular dichroism spectroscopic methods. The circular dichroism spectra of these three types of G-quadruplex DNA all showed major positive absorption peaks at ca. 290 nm with shoulder peaks at ca. 270 nm, along with a negative absorption peak at ca. 250 nm. The presence of hemin did not induce obvious circular dichroism spectral changes of these G-quadruplex DNA, which indicates that hemin is able to stabilize human telomeric antiparallel G-quadruplex DNA structure. For comparison, the addition of hemin induced obvious increment on the characteristic antiparallel circular dichroism absorption of the corresponding single-chain human telomeric sequences, which suggests that hemin may induce and stabilize the formation of antiparallel G-quadruplex DNA even in the absence of K+. On the other hand, the addition of the three types of G-quadruplex DNA into the hemin solution with gradually increasing concentrations induced significant hypochromicity (31.86-47.26 %) along with 11-23 nm of bathochromicity on the UV-visible absorption of hemin. From this result, it is suggested that the high binding affinity of hemin to antiparallel G-quadruplex DNA is based on the intensive intercalative binding mode of hemin to antiparallel G-quadruplex DNA by the pi-pi stacking between the macrocyclic aromatic hemin and the guanine quartet. Èë²ØºÅ:WOS:000325089600119 ÎÄÏ×ÀàÐÍ: Article ÓïÖÖ: English ×÷Õ߹ؼü´Ê: G-quadruplex DNA; Hemin; Telomeric DNA; Intercalative binding KeyWords Plus: INHIBITOR; COMPLEXES; SEQUENCE; BINDING; LIGAND ͨѶ×÷ÕßµØÖ·: Liu, YC (ͨѶ×÷Õß) Guangxi Normal Univ, State Key Lab Cultivat Base Chem & Mol Engn Med R, Sch Chem & Chem Engn, Guilin 541004, Peoples R China. µØÖ·: |
3Â¥2013-12-20 16:35:07
baiyuefei
°æÖ÷ (ÎÄѧ̩¶·)
·çÑ©
- LS-EPI: 1647
- Ó¦Öú: 4642 (¸±½ÌÊÚ)
- ¹ó±ö: 46.97
- ½ð±Ò: 658598
- É¢½ð: 11616
- ºì»¨: 995
- ɳ·¢: 81
- Ìû×Ó: 69427
- ÔÚÏß: 13331.5Сʱ
- ³æºÅ: 676696
- ×¢²á: 2008-12-18
- ÐÔ±ð: GG
- רҵ: ºÏ³ÉÒ©Îﻯѧ
- ¹ÜϽ: Óлú½»Á÷
4Â¥2013-12-20 16:35:23














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