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wilzu

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[求助] Block the 3' end of a DNA oligo

金币不多,求各位帮忙。
I want to irreversibly block the 3’ end of a DNA oligo so that it cannot be removed by the 3’-to-5’ exonuclease activity of proof-reading DNA polymerases (e.g. Platinum Pfx or Phusion Hot Start II). I have tried inverted dT, but it gets removed.

Is there a way to block the 3’ end of a DNA oligo so it can withstand thermocycling in the presence of a proof-reading polymerase?

Thanks,
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