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The best extraction solvents for simultaneously determination of chlorophyll a and b and carotene were methanol and acetone. For cherry tomatoes and tomatoes the best extraction solvent was methanol for chlorophyll a and acetone for carotene. For peppers the best extraction solvent was acetone and for cucumbers is methanol; Variation coefficients were high, both between vegetable species and between tested varieties or hybrids. Determination of the three pigments from different vegetables can be provided with spectrophotometric method using methanol as extractant. Beacause the methanol is toxic,extraction with acetone is preferred, providing reliable results that must be corrected with a suitable coefficient according to the vegetable species. |
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stingdhk
铁杆木虫 (著名写手)
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xiehuaiyan: 金币+30, 翻译EPI+1, ★★★很有帮助 2013-11-08 12:36:24
xiehuaiyan: 金币+30, 翻译EPI+1, ★★★很有帮助 2013-11-08 12:36:24
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The best extraction solvents for simultaneously determination of chlorophyll a and b and carotene were methanol and acetone. For cherry tomatoes and tomatoes the best extraction solvent was methanol for chlorophyll a and acetone for carotene. For peppers the best extraction solvent was acetone and for cucumbers is methanol; Variation coefficients were high, both between vegetable species and between tested varieties or hybrids. Determination of the three pigments from different vegetables can be provided with spectrophotometric method using methanol as extractant. Beacause the methanol is toxic,extraction with acetone is preferred, providing reliable results that must be corrected with a suitable coefficient according to the vegetable species. 甲醇和丙酮是同时测定叶绿素a，b和胡萝卜素的最佳提取溶剂。对于樱桃番茄和番茄的最佳提取是用甲醇提取叶绿素a，丙酮提取胡萝卜素。辣椒和黄瓜的最佳提取溶剂分别为丙酮和甲醇。 不同品种蔬菜之间以及参试品种或杂交品种之间的变异系数都较高。 不同蔬菜中的三种颜料可以用甲醇作提取溶剂，通过分光光度法来测定。由于甲醇的毒性，因而我们优先选用丙酮提取，但需要根据蔬菜品种确定一个合适的校正系数，以提供准确可靠的结果。 |
2楼2013-11-08 10:24:14