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zhangaimeicz: 金币+10, ★★★★★最佳答案, 非常满意 2013-10-14 14:42:01
oven1986: 检索EPI+1, 感谢你对版块的支持！ 2013-10-14 18:26:29
恭喜，收录了
Accession number:
20115214649165
Title:
Comparative analysis of p53 and K-ras gene mutations in gastric cancer tissue
Authors:
Zhang, Aimei1 ; Sun, Kun1 ; Wang, Rong2 ; Xie, Hua2
Author affiliation:
1College of Life Science, Northwest Normal University, Lanzhou 730070, China
2State Base for Drug Clinical Trials, Lanzhou General Hospital of PLA, Lanzhou,730050, China
Corresponding author:
Sun, K. (kunsun@nwnu.edu.cn)
Source title:
Advanced Materials Research
Abbreviated source title:
Adv. Mater. Res.
Volume:
393-395
Monograph title:
Biotechnology, Chemical and Materials Engineering
Issue date:
2012
Publication year:
2012
Pages:
651-654
Language:
English
ISSN:
10226680
ISBN-13:
9783037853078
Document type:
Conference article (CA)
Conference name:
2011 International Conference on Biotechnology, Chemical and Materials Engineering, CBCME 2011
Conference date:
December 28, 2011 - December 29, 2011
Conference location:
Kunming, China
Conference code:
87902
Publisher:
Trans Tech Publications, P.O. Box 1254, Clausthal-Zellerfeld, D-38670, Germany
Abstract:
Mutations of p53 and K-ras gene have been proven to play important roles in human tumor progression. Point mutations of p53 gene located in codon 282 and K-ras gene located in codons 61 are both gene hot spot mutation regions in patients with gastric cancer. The polymerase chain reaction (PCR) fragments included codon 282 of p53 and codon 61 of K-ras gene. The PCR fragments were analyzed by single-strand conformation polymorphism (SSCP) combined with CE and polyacrylamide gel electrophoresis (PAGE). Forty-two gastric cancer patients were analyzed using two developed method. P53 gene mutation was found in 24 cases (57.2 %) and K-ras were 17 cases (40.5 %). The results showed that p53 and K-ras gene mutation in gastric cancer tissue is a usual event, and the SSCP-CE was feasible for mutation detection of p53 and K-ras gene in populations. Combined detection of p53 and K-ras gene mutations of gastric cancer tissue by PCR-SSCP has a certain value to diagnosis of gastric cancer.
Number of references:
7
Main heading:
Genes
Controlled terms:
Biotechnology - Capillary electrophoresis - Chemical analysis - Conformations - Diseases - Polymerase chain reaction - Tissue - Tissue engineering
Uncontrolled terms:
Gastric cancers - Gene mutation - K-ras - P53 - Single-strand conformation polymorphism
Classification code:
461 Bioengineering and Biology - 801 Chemistry - 804 Chemical Products Generally
DOI:
10.4028/www.scientific.net/AMR.393-395.651
Database:
Compendex |
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