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xiehuaiyan

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The research was conducted on 17 samples of several vegetable species collected from different greenhouses located in southern part of Romania. Investigated species were cherry tomatoes, common tomatoes, peppers and cucumbers, in several varieties and hybrids existing in culture.
Extraction of pigments was carried out in stoppered tubes. Vegetables samples were  prepared with a laboratory homogenizer using about 1 g fresh material. Three extraction solutions were used for each sample: 95% aqueous diethyl ether solution (petroleum ether), 90% aqueous methanol solution and 100% acetone. The extraction ratio was 1:50.
Homogenized mixture is separated by centrifugation at 3000 rpm, for 10 minutes.  
The analytical determination was performed with Helios α spectrophotometer at the
following wavelengths: 645, 653, 662 and 664 nm, for chlorophyll a and b (according to each extraction solvent) and 470 nm for carotene.
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xux730

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xiehuaiyan: 金币+15, 翻译EPI+1, ★★★很有帮助 2013-09-29 13:19:01
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