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The research was conducted on 17 samples of several vegetable species collected from different greenhouses located in southern part of Romania. Investigated species were cherry tomatoes, common tomatoes, peppers and cucumbers, in several varieties and hybrids existing in culture. Extraction of pigments was carried out in stoppered tubes. Vegetables samples were prepared with a laboratory homogenizer using about 1 g fresh material. Three extraction solutions were used for each sample: 95% aqueous diethyl ether solution (petroleum ether), 90% aqueous methanol solution and 100% acetone. The extraction ratio was 1:50. Homogenized mixture is separated by centrifugation at 3000 rpm, for 10 minutes. The analytical determination was performed with Helios α spectrophotometer at the following wavelengths: 645, 653, 662 and 664 nm, for chlorophyll a and b (according to each extraction solvent) and 470 nm for carotene. |
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xiehuaiyan: 金币+15, 翻译EPI+1, ★★★很有帮助 2013-09-29 13:19:01
xiehuaiyan: 金币+15, 翻译EPI+1, ★★★很有帮助 2013-09-29 13:19:01
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供参考： 该项目对从位于罗马尼亚南部不同暖棚的数个蔬菜品种中采集的17个样品进行研究。研究的养殖中数个不同及杂交的品种，包括樱桃西红柿、普通西红柿、辣椒和黄瓜。 色素提取在带塞的试管中进行。蔬菜样品用实验室专用匀浆机对1克左右的新鲜材料进行匀浆而制备。每个样品用三种萃取液萃取：95％乙醚水溶液（石油醚），90％甲醇水溶液和100％丙酮。抽提比例为1:50。 匀浆混合物通过离心（转速为3000rpm）10分钟而分离。 利用Helios α分光光度计分析测定叶绿素a和b（根据各自的萃取溶剂）（波长：645，653，662和664 nm）的溶度和胡萝卜素（波长470 nm）的溶度。 |
2楼2013-09-26 15:58:06