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liuweipeng银虫 (小有名气)
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[求助]
请问melting curves的图怎么做
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最近投文章，审稿人问了个问题，说我melting curves的斜率太大，我找了很多文献终于发现问题所在，但不知道怎么改。 我做的图大约像这张图A的样子，我估计审稿人看的都是图B的样子。这篇文章中图B是图A标准化后的结果。请问这两张图怎么互转，所谓的标准化是什么意思。 图例说明：Figure 3. Genotyping of two adjacent single-nucleotide polymorphisms (HbS and HbC) within a 110-bp β-globin fragment with use of high-resolution amplicon melting curve analysis. The original high-resolution melting curves (A) include two different individuals for each of the six different genotypes, each individual run in duplicate. (B), the same curves as in panel A after normalization. (C), normalized curves after temperature-shifting of each curve to overlay them on one of the AA curves between 5% and 10% fluorescence. (D), fluorescence difference curves obtained by subtracting each curve in panel C from one wild-type (AA) curve (for clarity, the other AA samples are not shown). Because of the shift in temperature of the curves in panels C and D, the temperature axis no longer reflects absolute temperatures but rather reflects temperature differences relative to the superimposed segments of the curves.  F3.large.jpg |
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