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The aim of the research was to determine the chlorophyll and carotene pigments
simultaneously from different vegetables, using three organic solvents, in order to obtain the best extraction solution. Absorptive properties of the pigments were followed up in quantitative analysis using a spectrophotometric method. The literature presents two methods to determine these pigments - the trichromatic method and monochromatic method, the two methods differing by the used wavelengths. It is known that chlorophyll pigments have broad absorption band from blue to red. Also the coextracted carotenoids have maximum absorption in blue band so that the chosen determination method is based on measuring the absorbance at three wavelengths for each type of matrix and then calculate chlorophyll and carotene using
the three "trichromatic equations" of LICHTENTALER and WELLBURN

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The aim of the research was to determine the chlorophyll and carotene pigments simultaneously from different vegetables, using three organic solvents, in order to obtain the best extraction solution.

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Absorptive properties of the pigments were followed up in quantitative analysis using a spectrophotometric method.
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The literature presents two methods to determine these pigments - the trichromatic method and monochromatic method, the two methods differing by the used wavelengths.
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It is known that chlorophyll pigments have broad absorption band from blue to red.
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Also the coextracted carotenoids have maximum absorption in blue band so that the chosen determination method is based on measuring the absorbance at three wavelengths for each type of matrix and then calculate chlorophyll and carotene using the three "trichromatic equations" of LICHTENTALER and WELLBURN
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