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指尖上的泪

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[求助] 磷酸氢二钠柠檬酸缓冲溶液

One  gram of konjac powder was added to  50ml 0.05 M Na2HPO4–citric acid buffer and then mixed with neutral ?-mannanase (E/S ranging from 0.01  to  0.7,w/w) to start the reaction.The mixture was incubated at pH 2.0–10 for reaction times rang-ing from 0  to  7  h, while the temperature of the water bath was kept steady at  a  given temperature (reaction temperature ranged  from 20 to 80◦C). The reaction was stopped by  boiling the sam-ples for 15 min and then
the samples were centrifuged at  6000 rpm(2535× g). The supernatant was then tested for DRS and TRS and the DP was calculated.这个中间的加入磷酸氢二钠柠檬酸缓冲溶液的目的是什么?
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