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lgs1

木虫 (小有名气)

[求助] 引物设计

我有一段基因序列,里面A和T的含量很高,请帮我设计一个检测用引物吧,80~200bp左右,蛋白来源是金黄色葡萄球菌,原核
GTGGAACGTACATTTTTAATGATTAAACCAGACGCAGTACAAAGAAATCTAATTGGTGAAGTAATTTCAAGAATTGAAAGAAAAGGACTAAAACTTGTCGGTGGTAAATTAATGCAAGTACCAATGGAACTTGCTGAAACACATTATGGTGAACACCAAGGTAAATCATTTTATAATGATTTAATTTCATTTATTACATCAGCACCAGTGTTCGCAATGGTAGTTGAAGGTGAAGATGCAGTTAATGTATCTAGACATATTATTGGCAGCACCAATCCTTCAGAAGCTTCACCAGGATCAATTAGAGGTGATTTAGGTTTAACTGTTGGTAGAAATATCATTCACGGTTCAGATTCATTAAAGTCTGCTGAACGTGAAATTAATTTATGGTTTAATGAAAATGAAATTACTAGCTATGCTTCACCACGTGATGCTTGGTTATACGAATAA
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huanghznd

金虫 (小有名气)

【答案】应助回帖

★ ★ ★ ★ ★ ★
感谢参与,应助指数 +1
1949stone: 回帖置顶 2013-07-17 11:07:19
1949stone: 金币+3, good 2013-07-17 11:07:35
lgs1: 金币+3, ★★★很有帮助 2013-07-17 19:39:16
#5: Product of length 104 (rating: 171)
      Contains region of the molecule from 16 to 119
      Tm: 66.0 C  TaOpt: 44.5 C  GC: 30.8
    Sense Primer:
      TTAATGATTAAACCAGACGC
      Similarity: 100.0%
      Length: 20  Tm: 44.9 C  GC: 35.0
      dH: -152.3 kcal/mol  dS: -401.7 cal/mol  dG: -30.8 kcal/mol
    Antisense Primer:
      ACTTGCATTAATTTACCACC
      Similarity: 100.0%
      Length: 20  Tm: 43.9 C  GC: 35.0
      dH: -152.5 kcal/mol  dS: -403.7 cal/mol  dG: -30.4 kcal/mol
    Tm Difference: 1.1
    GC Difference: 0.0
2楼2013-07-17 11:04:53
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筱筱毛毛虫

铜虫 (初入文坛)

【答案】应助回帖

★ ★
gyesang: 金币+2, 鼓励回帖应助! 2013-08-04 00:13:49
最简单的方式 blast premier! http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome  参数可以自己设计了 !
3楼2013-08-03 14:18:07
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